The PYR reagent is used for detection of Pyrrolidonyl arylamidase enzyme activity of ß-hemolytic Streptococci.The chromogenic mixture of PYR Agar (M1489) serves as a substrate for PYR enzyme. The PYR reagent reacts with ß-naphthylamine to form a red coloured Schiff’s base indicating a positive reaction.Escherichia coli ATCC 25922 Negative reaction
Enterococcus faecalis ATCC 29212 Positive reaction (red colouration around colony)