Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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PSB HiVeg™ Broth Base
As low as
$225.50
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Availability: In stock
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SKU:
MV941
For primary enrichment and enumeration of Yersinia enterocolitica from food.
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Intended Use
PSB HiVeg Broth Base is recommended for primary enrichment and enumeration of Yersinia enterocolitica from food.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 20.0 |
| Sodium chloride | 10.0 |
| Disodium phosphate, anhydrous | 18.0 |
| Monopotassium phosphate, monohydrate | 3.0 |
Final pH (at 25°C) 7.5 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV941 | M941 |
| HiVeg peptone | Peptic digest of animal tissue |
Recommended for
Primary enrichment and enumeration of Yersinia enterocolitica from foods.
Reconstitution
50.60 g/l
Quantity on preparation (500g)
9.88 L
pH (25°C)
7.5 ± 0.2
Supplement
Cycloheximide
Sterilization
121°C / 15 minutes.
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 50.60 grams in 1000 ml distilled water. Heat if necessary to ensure complete solution. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to room temperature and aseptically add filter sterilized Cycloheximide solution to a final concentration of 100 mg/litre medium.
Principle and Interpretation
PSB HiVeg Broth Base is prepared by using HiVeg peptone in place of Peptic digest of animal tissue which makes the medium free from BSE/TSE risks. PSB HiVeg Broth is a modification of PSB Broth recommended by APHA (1) for enrichment of Yersinia species.
HiVeg peptone provides essential growth nutrients. Sodium chloride maintains osmotic equilibrium while phosphates buffer the medium well.
The food homogenate is added to primary enrichment broth and is held at refrigeration temperature of 4°C for 1-4 weeks and at one or more intervals, aliquots of the homogenate are inoculated directly onto selective agar and/or into selective broth, which following incubation is streaked onto selective agar media like MacConkey HiVeg Agar (MV081), SS HiVeg Agar (MV081), Yersinia Selective HiVeg Agar Base (MV843).
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge homogeneous, free flowing powder.
Colour and Clarity
Light yellow coloured, clear to slightly hazy solution.
Reaction
Reaction of 5.06% w/v aqueous solution is pH 7.5 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 3-5 days or at refrigeration temperature for 4-7 days.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Yersinia enterocolitica (27729) | 102-103 | good-luxuriant |
| Yersinia pseudotuberculosis (29833) | 102-103 | good-luxuriant |
References
- Downes FP and Ito K (Eds.), 2001, Compendium of Methods For The Microbiological Examination of Foods, 3rd ed., APHA, Washington, D.C.
| Product Name | PSB HiVeg™ Broth Base |
|---|---|
| SKU | MV941 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Speck M. (Ed.), 1984, Compendium of Methods for The Microbiological Examination of Foods, 3rd ed., APHA, Washington,D.C. |
| Customized Product Available | No |
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