Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Standard Nutrient HiVeg™ Agar
As low as
$232.10
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Availability: In stock
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SKU:
MV877
A general utility medium for cultivation and enumeration of not particularly fastidious microorganisms.
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Standard Nutrient HiVeg™ Agar
Standard Nutrient HiVeg Agar is used as a general utility medium for cultivation and enumeration of not particularly fastidious microorganisms.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone No. 1 | 10.0 |
| HiVeg extract | 10.0 |
| Sodium chloride | 5.0 |
| Agar | 20.0 |
Final pH (at 25°C) 7.6 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV877 | M877 |
| HiVeg peptone No. 1 | Lean meat |
| HiVeg extract | Beef extract |
Recommended for
Cultivation and enumeration of not particularly fastidious microorganisms.
| Reconstitution | 45.0 g/l |
|---|---|
| Quantity on preparation (500g) | 11.11 L |
| pH (25°C) | 7.6 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Directions
Suspend 45 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Standard Nutrient HiVeg Agar is prepared by using HiVeg peptone No. 1 and HiVeg extract which are of vegetable origin and free from BSE/TSE risks. Standard Nutrient HiVeg Agar is the modification of Standard Nutrient Agar which is formulated as per the recommendation of APHA as a general purpose medium for the cultivation of non-fastidious organisms from water and wastewater, dairy and food products (1, 2).
HiVeg peptone No. 1 provides amino acids and large chain peptides. HiVeg extract provides water soluble substances like carbohydrates, vitamins, organic nitrogen compounds and salts. Sodium chloride maintains osmotic equilibrium.
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 2.0% Agar gel.
Colour and Clarity
Light amber coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 4.5% w/v aqueous solution is pH 7.6 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli (25922) | 102-103 | good-luxuriant | >70% |
| Pseudomonas aeruginosa (27853) | 102-103 | good-luxuriant | >70% |
| Salmonella serotype Typhi (6539) | 102-103 | good-luxuriant | >70% |
| Staphylococcus aureus (25923) | 102-103 | good-luxuriant | >70% |
| Streptococcus pneumoniae (6303) | 102-103 | good-luxuriant | >70% |
| Product Name | Standard Nutrient HiVeg™ Agar |
|---|---|
| SKU | MV877 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Greenberg A. E., Trussell R. R. and Clesceri L. S. (Eds.), 1985, Standard Methods for the Examination of Water andWastewater, 16th ed., APHA, Washington, D.C. |
| Customized Product Available | No |
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