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Wilkins Chalgren Anaerobic HiVeg™ Agar Base
As low as
$268.40
Availability: Out of stock
Availability: In stock
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SKU:
MV832
Used for selective isolation and cultivation of anaerobic bacteria and susceptibility testing of anaerobes by the agar dilution method.
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Intended Use
Recommended for the selective isolation and cultivation of anaerobic bacteria and also for susceptibility testing of anaerobic bacteria by the agar dilution method..
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™™ hydrolysate | 10.000 |
| HiVeg™™ peptone | 10.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 1.000 |
| Sodium chloride | 5.000 |
| L-Arginine | 1.000 |
| Sodium pyruvate | 1.000 |
| Ferric pyrophosphate | 0.005 |
| Menadione | 0.0005 |
| Agar | 10.000 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 43.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C before adding antibiotics to be tested. Mix gently and pour into sterile Petri plates. For cultivation of anaerobes, aseptically add the rehydrated contents of 2 vials each of Anaero Supplement (FD001) or G.N. Anaero Supplement (FD002) as desired into sterile molten medium before pouring into sterile Petri plates.
Principle And Interpretation
Anaerobic bacteria are widespread in soil, marshes, lake and river sediments, oceans, sewage, food and animals. In humans, anaerobic bacteria normally are prevalent in the oral cavity around the teeth, in the gastrointestinal tract, in the orifices of the genitourinary tract and on the skin. Anaerobic infections in humans and various animals can involve virtually any organ under immunocompromised conditions (1). Also, anaerobic infections are often associated with tissue necrosis and abscess formation, leading to impaired delivery of antimicrobial agents in blood to the actual site of infection. This explains why anaerobic infections are often aggressively managed with debridement, aspiration and/or surgical removal of infected tissue. Because of the technical and interpretive difficulties associated with anaerobic susceptibility testing, presentation of definitive recommendations is difficult (2). Wilkins Chalgren Anaerobic Agar Base, formulated by Wilkins and Chalgren (3), along with Brucella Agar Base is the preferred medium for agar dilution tests with anaerobes. This medium is also recommended for testing anaerobic bacteria (4,5,6). Wilkins Chalgren Agar need to be appropriately supplemented to support the growth of certain anaerobic bacteria. Wilkins Chalgren Anaerobic HiVeg™™ Agar Base is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. Ferric pyrophosphate and Menadione (Vitamin K3) enhances the growth of Bacteroides species and Prevotella melaninogenica, respectively and many other species of gram-negative anaerobic rods (5,7). The medium can also be supplemented with defibrinated or lysed blood for the growth of fastidious anaerobic bacteria (8). HiVeg™ hydrolysate and HiVeg™™ peptone serve as sources of essential nutrients including carbon andnitrogen. Yeast extract provides vitamins and other growth factors like purines and pyrimidines that are essential for the growth of P. melaninogenica. Arginine serves as an amino acid source while pyruvate serves as an energy source. The medium can be made selective for non-sporing anaerobic bacteria and gram-negative anaerobic bacteria by addition of Anaero Supplement (FD001) and G. N. Spore Anaerobic Supplement (FD002) respectively.
Type of specimen
Please add specimens
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Proper anaerobic conditions must be maintained for optimal recovery of organisms
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.0% Agar gel.
Colour and Clarity of prepared medium
Medium amber coloured clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 4.3% w/v aqueous solution at 25°C. pH: 7.1±0.2
pH
6.90-7.30
Cultural Response
Cultural characteristics observed with added Anaero Supplement(FD001) or G.N. Anaero Supplement (FD002) under anaerobic condition, after an incubation at 35-37°C of 48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Bacteroides fragilis ATCC 25285 | 50-100 | luxuriant | >=50% |
| Clostridium perfringens ATCC 12924 | 50-100 | luxuriant | >=50% |
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | 0% |
| Prevotella melaninogenicus ATCC 15930 | 50-100 | luxuriant | >=50% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (9,10).
| Product Name | Wilkins Chalgren Anaerobic HiVeg™ Agar Base |
|---|---|
| SKU | MV832 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Koneman E. W., Allen S. D., Janda W. M., Schreckenberger P. C., Winn W. C. Jr., 1992, Colour Atlas and Textbook ofDiagnostic Microbiology, 4th Ed., J. B. Lippinccott Company.2.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical Microbiology,ASM, Washington, D.C.3.Wilkins T. D. and Chalgren S., 1976, Antimicrob. Agents Chemother., 10 : 926.4.King A., Phillips I., 1988, J. Antimicrob. Chemother., 21:425-438.5.Clinical and Laboratory Standards Institute, 2006, Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria,Approved standard M11-A3, CLSI, Villanova, Pa.6.Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and McCartney Practical Medical Microbiology,1996, 14th Edition, Churchill Livingstone.7.Gibbons R. J. and MacDonald J. B., 1960, J. Bacteriol., 80:164.8.Isenberg (Ed.),2004, Clinical Microbiology Procedures Handbook, Vol.3, American Society for Microbiology, Washington.D.C. |
| Customized Product Available | No |
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