Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Glucose Yeast HiVeg™ Peptone Agar
As low as
$239.80
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV757
Used for isolation of yeasts from soil specimens.
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Intended Use
Isolation of yeast from soil specimens.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 10.00 |
| Yeast extract | 5.00 |
| Dextrose | 20.00 |
| Agar | 15.00 |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 50 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by completely replacing animal based peptones with vegetable peptones. Glucose Yeast HiVeg Peptone Agar is modification of Glucose Yeast Peptone Agar formulated as described by Subba Rao (1) for isolating yeasts from soil specimens. This is a highly nutritious medium which may be used not only for isolating yeasts but also some fastidious microorganisms. HiVeg peptone and yeast extract provide nitrogenous nutrients especially the amino acids and peptides. Yeast extract also supplies Vitamin B. Dextrose added in the medium is a readily available source of energy and a good carbohydrate source for yeasts.
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and clarity
Light to medium amber coloured, slightly opalescent gel forms in petri plates.
Product Profile
- Vegetable based (Code MV)
- MV757 (HiVeg peptone)
- Animal based (Code M)
- M757 (Peptic digest of animal tissue)
- Reconstitution
- 50.0 g/l
- Quantity on preparation (500g)
- 10.0 L
- pH (25°C)
- -
- Supplement
- None
- Sterilization
- 121°C / 15 minutes.
- Storage
- Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Cultural Response
Cultural characteristics observed after an inubation at 30°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Saccharomyces cerevisiae (9763) | 102-2x102 | luxuriant | >70% |
| Saccharomyces uvarum (9080) | 102-2x102 | luxuriant | >70% |
References
- Subba Rao N.S., 1977, 'Soil Microorganisms and Plant Growth', Oxford and IBH Publishing Co., India.
| Product Name | Glucose Yeast HiVeg™ Peptone Agar |
|---|---|
| SKU | MV757 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Subba Rao N. S., 1977, Soil Microorganisms and Plant Growth, Oxford and IBH Publishing Co., New Delhi. 2.Ausubel, Brent, Kingston, Moore, Seidman, Smith and Struhl, 1994, Current Protocols in Molecular Biology, CurrentProtocols, Brooklyn, N.Y. |
| Customized Product Available | No |
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