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Enriched Thioglycollate HiVeg™ Broth
As low as
$248.60
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV738
Recommended for isolation, cultivation and identification of a wide variety of obligate anaerobic bacteria.
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Intended Use
Recommended for isolation, cultivation and identification of a wide variety of obligate anaerobic bacteria.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate | 17.000 |
| Soya peptone | 3.000 |
| Dextrose (Glucose) | 6.000 |
| Sodium chloride | 2.500 |
| Sodium thioglycollate | 0.500 |
| L-Cystine | 0.250 |
| Sodium sulphite | 0.100 |
| Ferric pyrophosphate | 0.005 |
| Vitamin K1 | 0.0001 |
| Agar | 0.700 |
| Sodium bicarbonate | 1.000 |
| Final pH (at 25°C) | 7.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 31.06 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at A 118 - 121°C for 15 minutes. Aseptically add 10% rabbit or horse serum. Cool and dry under 85% Nitrogen (N2) + 10% Hydrogen (H2) + 5% Carbon dioxide (CO2) atmosphere. A corresponds to 12 - 15 lbs pressure respectively.
Principle And Interpretation
Enriched Thioglycollate Medium is recommended for use in isolation and cultivation of fastidious and obligate anaerobic bacteria from clinical materials (1). This medium is often used for susceptibility testing of anaerobes by broth disk elution method. This medium is the modification of original Brewers formulation (2,3), with the addition of vitamin K1, sodium bicarbonate, hemin and rabbit or horse serum. Enriched Thioglycollate HiVeg™ Broth is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ hydrolysate and soya peptone supports growth of wide variety of fastidious microorganisms. Sodium thioglycollate lowers the oxidation-reduction potential for anaerobic growth and also neutralizes the bacteriostatic effect of mercurial compounds. Most organisms show earlier and more vigorous growth in presence of dextrose, Ferric pyrophosphate and vitamin K1. Hemin is the source of X-factor, which stimulates the growth of many microorganisms.
Type of specimen
Please add specimens
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- The tubes should not be reheated as frequent boiling leads to development of toxic products.
- Prior to use the medium should be boiled once to remove the absorbed oxygen.
- Before inoculation, the tubes should be brought to room temperature.
- The medium should not be used in fermentation process as medium contains yeast extract which is high in carbohydrate content.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light amber coloured, clear to slightly opalescent solution in tubes
Reaction: Reaction of 3.0% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH: 6.80-7.20
Cultural Response: Cultural characteristics observed under anaerobic condition, after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Phocaeicola vulgatus ATCC 8482 | 50-100 | luxuriant |
| Clostridium perfringens ATCC 12924 | 50-100 | luxuriant |
| Clostridium sporogenes ATCC 11437 | 50-100 | luxuriant |
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant |
*- Formerly known as Bacteroides vulgatus
Storage and Shelf Life
Store between 10-30°C in tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period..
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Enriched Thioglycollate HiVeg™ Broth |
|---|---|
| SKU | MV738 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Allen S. D., Siders J. A. and Movler M., 1985, In Manual of Clinical Microbiology, Lennette, Balows, Hausler and Shadomy(Eds.), 4th Ed., ASM, Washington, D.C. 2.Brewer J. H., 1940 and 1943, J. Bacteriol., 39:10 and 46:395. 3.Brewer J. H., 194 3.J. Bacteriol., 46:395. |
| Customized Product Available | No |
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