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Moeller Decarboxylase HiVeg™ Broth w/ Lysine HCl
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$179.30
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Availability: In stock
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SKU:
MV687
Used for differentiation of bacteria on the basis of their ability to decarboxylate L-Lysine hydrochloride.
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Product Profile
Vegetable based (Code MV)
MV393/MV687/MV688/MV689
HiVeg peptone
HiVeg extract
Animal based (Code M)
M393/M687/M688/M689
Peptic digest of animal tissue
Beef extract
Recommended for: Differentiating bacteria on the basis of their ability to decarboxylate the amino acids.
Reconstitution:
(MV393): 10.52 g/l
(MV687/MV688/MV689): 20.52 g/l
Quantity on preparation:
(500g): (MV393): 47.52 L
(100g): (MV393): 9.50 L
(100g): (MV687/MV688/MV689): 4.87 L
pH (25°C): 6.0 ± 0.2
Supplement: (MV393): Amino acids.
Sterilization: 121°C / 10 minutes
Storage: Dry Medium-Below 30°C, Prepared Medium 2 - 8°C.
Composition **
| Ingredients | MV393 Grams/Litre |
MV687 Grams/Litre |
MV688 Grams/Litre |
MV689 Grams/Litre |
|---|---|---|---|---|
| HiVeg peptone | 5.00 | 5.00 | 5.00 | 5.00 |
| HiVeg extract | 5.00 | 5.00 | 5.00 | 5.00 |
| Dextrose | 0.50 | 0.50 | 0.50 | 0.50 |
| Bromo cresol purple | 0.01 | 0.01 | 0.01 | 0.01 |
| Cresol red | 0.005 | 0.005 | 0.005 | 0.005 |
| Pyridoxal | 0.005 | 0.005 | 0.005 | 0.005 |
| L-Lysine hydrochloride | — | 10.00 | — | — |
| L-Ornithine hydrochloride | — | — | 10.00 | — |
| L-Arginine hydrochloride | — | — | — | 10.00 |
Final pH (at 25°C) 6.0 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 10.52 grams of MV393 and 20.52 grams of MV687/MV688/MV689 in 1000 ml distilled water. Add 10 gm. of L-Lysine, L-Arginine, L-Ornithine or other L-amino acids in MV393. When using DL-amino acids, use 2% concentration. Heat if necessary to dissolve the medium completely. When L-Ornithine is added, readjustment of the pH is required. Dispense in 5 ml amount in screw-capped tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes. Suspend 20.52 grams of MV687 or MV688 or MV689 in 1000 ml distilled water and sterilize as mentioned above.
Principle and Interpretation
These media are used for differentiating gram-negative enteric bacilli on the basis of their ability to decarboxylate amino acids. The Decarboxylase Broth was introduced by Moeller for detecting the production of lysine and ornithine decarboxylase and arginine dihydrolase (1). Prior to Moeller's work, bacterial amino acid decarboxylases were studied by Gale (2) and Gale and Epps (3). These HiVeg media are prepared by replacing animal based peptones with vegetable peptones which are BSE/TSE risks free. Production of ornithine decarboxylase is a helpful criterion in differentiating Klebsiella and Enterobacter species. Klebsiella are non-motile and do not produce ornithine decarboxylase while Enterobacter are motile and produce ornithine decarboxylase except Enterobacter agglomerans (4).
These media contain HiVeg extract and HiVeg peptone which provide nitrogenous nutrients for the growth of bacteria. Dextrose is the fermentable carbohydrate and pyridoxal is the co-factor for the decarboxylase enzyme. Bromo cresol purple and cresol red are the pH indicators in the medium. When the medium is inoculated with the dextrose fermenting bacteria, the pH is lowered due to acid production which changes the colour of the indicator from purple to yellow. Acid produced stimulates decarboxylase enzyme. Decarboxylation of lysine yields cadaverine while putrescine is produced due to ornithine decarboxylation. Arginine is first hydrolyzed to ornithine which is then decarboxylated to form putrescine. Formation of these amines increases the pH of the medium, changing the colour of the indicator from yellow to purple. If the organisms do not produce the appropriate enzyme, the medium remains acidic, yellow in colour. Each isolate to be tested should also be inoculated into the basal medium tube lacking the amino acid.
Inoculated tubes must be protected from air with a layer of sterile mineral oil. Exposure to air may cause alkalinization at the surface of the medium which makes the test invalid.
Quality Control
Appearance of powder
Greenish yellow coloured, homogeneous, free flowing powder.
Colour and Clarity
Purple coloured, clear solution without any precipitate.
Reaction
Reaction of 1.05% w/v of MV393 or 2.05% w/v of MV687/MV688/MV689 aqueous solution is pH 6.0 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after inoculating tubes, overlaying with sterile mineral oil and incubating at 35 - 37°C for upto 4 days.
| Organisms (ATCC) | Lysine | Arginine | Ornithine |
|---|---|---|---|
| Citrobacter freundii (8090) | + | — | + |
| Enterobacter aerogenes (13048) | + | — | + |
| Escherichia coli (25922) | + | — | + |
| Klebsiella pneumoniae (13883) | — | ± | — |
| Proteus vulgaris (13315) | — | — | — |
| Proteus mirabilis (25933) | — | + | + |
| Pseudomonas aeruginosa (9027) | — | + | + |
| Salmonella serotype Paratyphi A | + | (+) or + | + |
| Salmonella serotype Typhi (6539) | + | (+) or + | — |
| Shigella flexneri (12022) | — | or (+) | — |
| Shigella sonnei (25931) | — | ± | — |
| Shigella dysenteriae (13313) | — | or (+) | — |
| Serratia marcescens (8100) | + | — | + |
Key:
- + = positive reaction, purple colour
- — = negative reaction, yellow or no colour change
- ± = variable
- (+) = delayed positive reaction
MV689 Moeller Decarboxylase HiVeg Broth with Arginine Hydrochloride
- Control
- Citrobacter freundii
- Enterobacter aerogenes
- Escherichia coli
- Klebsiella pneumoniae
- Proteus vulgaris
- Pseudomonas aeruginosa
- Salmonella serotype Typhi
- Shigella flexneri
| Product Name | Moeller Decarboxylase HiVeg™ Broth w/ Lysine HCl |
|---|---|
| SKU | MV687 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Koneman E. W., Allen S. D., Janda W.M., Schreckenberger P. C., Winn W. C. Jr., 1992, Colour Atlas and Textbook ofDiagnostic Microbiology, 4th Ed., J. B. Lippinccott Company 2.Moeller V., 1955, Acta Pathol. Microbiol. Scand. 36:158. 3.Gale G. F., 1940, Biochem. J., 34:392. 4.Gale and Epps, 1943, Nature, 152:327. 5.Isenberg (Ed.), 1992, Clinical Microbiology Procedures Handbook, Vol. I, ASM, Washington, D. C. 6.FDA Bacteriological Analytical Manual, 8th Ed., AOAC International, Gaithersburg, Md. 7.Eaton A. D., Clesceri L. S. and Greenberg A. E., (Ed.), 1998, Standard Methods for the Examination of Water and Wastewater, 20th Ed., American Public Health Association, Washington, D.C |
| Customized Product Available | No |
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