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KG HiVeg™ Agar Base

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SKU:
MV658
Recommended for promoting fast and free sporulation to distinguish between Bacillus cereus and Bacillus thuringiensis, recommended by APHA .

Description

Kim-Goepfert (KG) HiVeg Agar Base with added supplements is used for promoting fast and free sporulation of Bacillus cereus and Bacillus thuringiensis.

Composition

Ingredients Grams/Litre
HiVeg peptone 1.0
Yeast extract 0.5
Phenol red 0.025
Agar 18.0
Final pH (at 25°C) 6.8 ± 0.2

Formula adjusted, standardized to suit performance parameters.

Product Profile

Vegetable based (Code MV)

  • MV658
  • HiVeg peptone

Animal based (Code M)

  • M658
  • Peptic digest of animal tissue

Recommended for : Promoting fast and free sporulation of Bacillus cereus and Bacillus thuringiensis.

Reconstitution : 19.53 g/l

Quantity on preparation (500g) : 25.6 L

pH (25°C) : 6.8 ± 0.2

Supplement : Polymyxin B Selective Supplement (FD003) and Egg Yolk Emulsion (FD045)

Sterilization : 121°C / 15 minutes.

Storage : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.

Directions

Suspend 19.53 grams in 900 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 100 ml sterile, Egg Yolk Emulsion (FD045) and reconstituted Polymyxin B Selective Supplement (FD003). Mix well and pour into sterile petri plates.

Principle and Interpretation

This medium is prepared by replacing Peptic digest of animal tissue by HiVeg peptone which is free from BSE/TSE risks. KG HiVeg Agar Base is the modification of KG Agar Base which was formulated by Kim and Goepfert (1) to promote free spore formation of Bacillus cereus and Bacillus thuringiensis within 20 - 24 hours.

HiVeg peptone and yeast extract supports growth and lecithinase production of Bacillus cereus, Bacillus thuringiensis. Bacillus polymyxa is unable to form lecithinase under the nutritionally poor conditions. Lecithinase activity is observed as opaque zone surrounding the individual colony. Bacillus cereus is resistant to Polymyxin B, where as gram-negative organisms are restricted by it. Bacillus cereus and Bacillus thuringiensis can be distinguished by means of microscopic examination, where the latter show endotoxin crystals in sporulated cells.

Quality Control

Appearance of powder
Light pink coloured, homogeneous free flowing powder.

Gelling
Firm, comparable with 1.8% Agar gel.

Colour and Clarity
Orange coloured, clear basal medium which on addition of sterile Egg Yolk Emulsion (FD045) and Polymyxin B Sulphate(FD003), forms orangish opalescent gel form in petri plates.

Reaction
Reaction of 1.95% w/v aqueous solution is pH 6.8 ± 0.2 at 25°C

Cultural Response

Cultural characteristics observed after an incubation at 30-35°C for 24 hours with added sterile Egg Yolk Emulsion (FD045) and Polymyxin B. Selective Supplement (FD003).

Organisms (ATCC) Inoculum (CFU) Growth Recovery Lecithinase
Bacillus cereus (11778) 102-103 luxuriant >50% +
Bacillus thuringiensis (10792) 102-103 good-luxuriant >50% +
Escherichia coli (25922) 102-103 none-poor <20% -

Key : + = Opaque zone around the colony.

More Information
Product Name KG HiVeg™ Agar Base
SKU MV658
Product Type HiVeg™
Physical Form Powder
Origin Animal Free (Veg)
Packaging type HDPE
References 1.Kim H. V. and Goepfert J. M., 1971, Appl. Microbiol., 22:581.
2.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C.
Customized Product Available No
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