Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Trichophyton HiVeg™ Agar-1
As low as
$254.10
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV531
Used for differentiation of Trichophyton species.
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Intended Use
Recommended for differentiation of Trichophyton species.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ acid hydrolysate, vitamin free | 2.500 |
| Dextrose (Glucose) | 40.000 |
| Potassium dihydrogen phosphate | 1.800 |
| Magnesium sulphate | 0.100 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 59.4 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Dispense in test tubes. Sterilize by autoclaving at 15 lbs pressure (121°) for 15 minutes. Allow the tubed medium to cool in a slanted position.
Principle And Interpretation
Nutritional tests were originally described by George and Camp (1) as an aid in the routine identification of Trichophyton species that seldom produce conidia or that resemble each other morphologically (1). Certain species have distinctive nutritional requirements, whereas others do not.
The method employs a Trichophyton HiVeg™ Agar-1 that is vitamin-free (Trichophyton HiVeg™ Agar-1, MV531) to which different vitamins are added i.e. inositol (Trichophyton HiVeg™ Agar-2, MV532), thiamine and inositol (Trichophyton HiVeg™ Agar-3, MV533), thiamine (Trichophyton HiVeg™ Agar-4) (MV534) and nicotinic acid (Trichophyton HiVeg™ Agar-5) (MV535). The method also employs an ammonium nitrate basal medium (Trichophyton Agar-6, M536) to which histidine is added (Trichophyton Agar-7, M152) (2). The various additives added help to determine the specific vitamin and amino acid requirements of the isolates. Trichophyton Agar-1 is used along with medium 2, 3 and 4 to determine whether the isolate require inositol, thiamine or both.
Trichophyton HiVeg™ Agar-1 is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. Nutritional requirements are determined by inoculating a control medium and a medium enriched with a specific vitamin or amino acid with Trichophyton isolates that have been presumptively identified by gross colony characteristics and microscopic morphology (2,3,4,5,6,7). Moderate to heavy growth in the vitamin or amino acid-enriched medium compared to little or no growth in the basal medium indicates that the isolate requires that nutrient.
Type of specimen
Isolated Microorganism
Specimen Collection and Handling:
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred to individual safety data sheets.
Limitations :
- Cultures contaminated with bacteria must be repeatedly grown on a medium containing antimicrobials such as BHI CC Agar, HiVeg™ (MV209). Many bacteria synthesize vitamins which may erroneous the results.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
White to light yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber coloured clear to slightly opalescent gel forms in tubes as slants
Reaction
Reaction of 5.94% w/v aqueous solution at 25°C. pH: 6.8±0.2
pH
6.60-7.00
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 2 weeks.
| Organism | Growth |
|---|---|
| Trichophyton equinum ATCC 22443 | none |
| Trichophyton mentagrophytes ATCC 9533 | good-luxuriant |
| Trichophyton rubrum ATCC 28191 | good-luxuriant |
| Trichophyton violaceum ATCC 24787 | none-poor |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).
| Product Name | Trichophyton HiVeg™ Agar-1 |
|---|---|
| SKU | MV531 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., 2.Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical Microbiology, ASM, Washington, D.C.George L. K., Camp L. B., 1957, J. Bacteriol., 74:113.Roberts G. D., 1985, In Washington (Ed.), Laboratory Procedures in Clinical Microbiology, 2nd Ed., Springer- Verlag, NewYork, N.Y. 4.Weitzman I., Rosenthal S. A. and Silva-Hutner M., 1988, In Wentworth (Eds.), Diagnostic Procedures for Mycotic andParasitic Infections, 7th Ed., American Public Health Association, Washington, D.C. 5.Haley L. D., Trandel J. and Coyle M. B., 1980, Cumitech 11, Practical methods for culture and identification of fungi in theclinical mycology laboratory, Coord. Ed., Sherris, American Society for Microbiology, Washington, D.C. 6.McGinnis M. R. and Pasarell L., 1992, In Isenberg (Ed.), Clinical Microbiology Procedures Handbook, Vol. 1, AmericanSociety for Microbiology, Washington, D.C. |
| Customized Product Available | No |
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