B.Q.Vaccine HiVeg™ Medium (Thioglycollate Broth w/ HiVeg™ Extract No. 2)

Principle And Interpretation

Anaerobic microorganisms have long been known as constituents of the normal bacterial flora of human and animal organisms. Both their pathogenic significance in medicine and their important role in food hygiene have, however, long been underestimated. During the past few years the importance of anaerobic microorganisms as pathogenic agents responsible for infectious diseases and the role they play in the microbial spoilage of food and water have been better appreciated. Extremely different spectra of anaerobic organisms are of importance for the examination of food and in the clinical microbiology. B.Q. Vaccine Medium (Thioglycollate Broth w/HL Extract) is modification of original Thioglycollate medium (1,2), recommended for the cultivation of anaerobic organisms on large scale. It is a nutritious medium due to the presence of HiVeg™ peptone, HiVeg™ infusion and HiVeg™ extract No.2. B.Q.Vaccine HiVeg™ Medium (Thioglycollate Broth w/ HiVeg™ Extract No. 2) is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. HiVeg™ peptone supply the nitrogenous compounds and growth factors. HiVeg™ infusion and HiVeg™ extract No.2 provide trace minerals, growth factors and vitamins for the growth of wide variety of organisms. Sodium thioglycollate acts as a reducing agent, which lowers the oxidation-reduction potential of the medium thereby enabling the obligate anaerobes to multiply. Added glucose, act as the source of energy. Dipotassium phosphate and sodium chloride helps in maintaining buffering action and isotonic conditions respectively in the medium.