Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Violet Red HiVeg™ Broth
As low as
$201.30
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV458
Recommended for detection and enumeration of coliforms from water and food.
Your enquiry has been submitted
Please login to Post an Enquiry.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 7.0 |
| Yeast extract | 3.0 |
| Sodium chloride | 5.0 |
| Synthetic detergent No. I | 1.5 |
| Lactose | 1.5 |
| Neutral red | 0.03 |
| Crystal violet | 0.002 |
Final pH (at 25°C) 7.4 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 26.53 grams in 1000 ml distilled water. Heat to boiling with stirring to dissolve the media completely. DO NOT AUTOCLAVE. If desired the media can be sterilized by autoclaving at 15 lbs pressure (121°C) for 15 minutes, dispense in tubes.
Principle and Interpretation
Violet Red HiVeg Broth is prepared by completely replacing animal based peptones by vegetable peptones which makes the medium free of BSE/TSE risks. Voilet Red HiVeg media like the conventional Violet Red Bile media is recommended by APHA for the detection and isolation of coliform organisms in water, milk, dairy and other food products (1,2,).
The medium is selective due to the presence of the inhibitors - synthetic detergent No.1 and crystal violet. Crystal violet inhibits gram-positive microorganisms especially Staphylococci. Neutral red is the pH indicator. Organisms which rapidly ferment lactose will produce pink to red colour (3). Lactose non-fermenters and late lactose fermenters produce pale colour. Other related gram-negative bacteria can be suppressed by incubation at >42°C or by anaerobic incubation. Incubation may be carried out at > 42°C for 18 hours, 32°C for 24-48 hours or 4°C for 10 days depending on the temperature characteristics of the organisms to be recovered (4).
Product Profile
Vegetable based (Code MV)
MV458
HiVeg peptone
Synthetic detergent No. I
Animal based (Code M)
M458
Peptic digest of animal tissue
Bile salts mixture
Recommended for: Isolation and detection of coliform organisms from water, milk and food.
Reconstitution: 26.53 g/l
Quantity on preparation (500g): 18.84 L
pH (25°C): 7.4 ± 0.2
Supplement: None
Sterilization: Boiling or 121°C / 15 minutes, if desired
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of powder: Pinkish beige coloured, homogeneous, free flowing powder.
Colour and Clarity: Reddish purple coloured, clear solution in tubes.
Reaction: Reaction of 2.65% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Colour of Medium |
|---|---|---|---|
| Enterobacter aerogenes (13048) | 10²-10³ | luxuriant | pinkish-red |
| Escherichia coli (25922) | 10²-10³ | luxuriant | pinkish red |
| Salmonella serotype Enteritidis (13076) | 10²-10³ | luxuriant | orangish-yellow |
| Staphylococcus aureus (25923) | 10²-10³ | inhibited | - |
References
- Frances Pouch Downes and Keith Ito (Eds.), 2001, Compendium of Methods For The Microbiological Examination of Foods, 4th ed., APHA, Washington, D.C.
- Standard Methods for the Examination of Dairy Products. 17th Edition, 2004 Edited by H. Michael Wehr and Joseph H.Frank.
- Davis J.G., 1951, Milk Testing, Dairy Industries Limited, London; pg 131
- Mossel D.A.A. and Vega C.L., 1973, Hith. Lab. Sci., 11:303.
| Product Name | Violet Red HiVeg™ Broth |
|---|---|
| SKU | MV458 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater,23rd ed., APHA, Washington, D.C 2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., American Public Health Association, Washington, D.C. 3.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition 4.Jorgensen,J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1. 5.MacConkey A., 1905, J. Hyg., 5, 333-37 6.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams and Wilkins, Baltimore 7.Marshall R. T., (Ed.), 1992, Standard Methods for the Examination of Dairy Products, 16th Ed., APHA, Washington, D. C. 8.Mossel D. A. A. and Vega C. L., 1973, Hlth. Lab. Sci., 11:303 9.Salfinger Y., and Tortorello M.L. , 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C. |
| Customized Product Available | No |
Write Your Own Review
Taurocholate Broth
As low as
$239.80
Violet Red Bile Broth
As low as
$201.30
Upsell Products
M-Endo Medium (without Membrane Filter)
As low as
$136.40
MacConkey Medium (without Membrane Filter)
As low as
$66.00
M-E.coli Medium (without Membrane Filter)
As low as
$66.00