Furunculosis HiVeg™ Agar

Principle and Interpretation

Furunculosis HiVeg Agar is prepared by using HiVeg hydrolysate in place of Casein enzymic hydrolysate which makes the medium free of BSE/TSE risks. Furunculosis HiVeg Agar is the modification of Furunculosis Agar which is formulated as per Griffin et al (1) for detection of Aeromonas salmonicida on the basis of production of brownish red pigment. Brownish red pigmentation within two to three days of incubation at 22°C is a positive presumptive evidence. For more rapid and distinct presumptive test, 0.5 ml of 1% aqueous solution of paraphenylenediamine can be added to the colonies of a 24 hours old culture growing on the surface of the agar slants and spread evenly over the full surafce of growth. The reagent should be in contact with the growth to visualize the reaction. After addition of the reagent, the tubes should be tipped and rotated to spread the reagent to cover the growth on slant, a deep purple colour is seen within 45 to 90 seconds.