Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Actidione HiVeg™ Agar w/ Actidione®
As low as
$778.80
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV400
For the enumeration and detection of bacteria in specimens containing large number of yeasts and moulds.
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Intended Use
Actidione HiVeg Agar with Actidione / Base without Actidione is used for the enumeration and detection of bacteria in specimens containing large number of yeasts and moulds.
Composition
| Ingredients | MV400 Grams/Litre | MV058 Grams/Litre |
|---|---|---|
| HiVeg hydrolysate | 5.00 | 5.00 |
| Yeast extract | 4.00 | 4.00 |
| Dextrose | 50.00 | 50.00 |
| Monopotassium phosphate | 0.55 | 0.55 |
| Potassium chloride | 0.425 | 0.425 |
| Calcium chloride | 0.125 | 0.125 |
| Magnesium sulphate | 0.125 | 0.125 |
| Ferric chloride | 0.0025 | 0.0025 |
| Manganese sulphate | 0.0025 | 0.0025 |
| Brom cresol green | 0.022 | 0.022 |
| *Actidione (Cycloheximide) | 0.01 | - |
| Agar | 15.00 | 15.00 |
Final pH (at 25°C) 5.5 ± 0.2
** Formula adjusted, standardized to suit performance parameters
Product Profile
Vegetable based (Code MV): MV400/MV058 HiVeg hydrolysate
Animal based (Code M): M400/M058 Casein enzymic hydrolysate
Recommended for
Enumeration and detection of bacteria in specimens containing large number of yeasts and moulds.
Reconstitution
(MV400): 75.26 g/l
(MV058): 75.25 g/l
Quantity on preparation (500g)
6.64 L
pH (25°C)
5.5 ± 0.2
Supplement
Cycloheximide for (MV058)
Sterilization
121°C / 15 minutes.
Storage
(MV400): Dry Medium and Prepared Medium 2 - 8°C.
(MV058): Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 75.26 grams of MV400 or 75.25 grams of MV058 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C. For (MV058) aseptically add 10 mg cycloheximide to the medium. Mix well before pouring into sterile petriplates.
Warning: *Actidione (Cycloheximide) is very toxic. Avoid skin contact or aerosal formation and inhalation.
Principle and Interpretation
This medium is prepared by replacing Casein enzymic hydrolysate with HiVeg hydrolysate. Actidione HiVeg Agar is the modification of Actidione Agar formulated by Green and Gray (1) which can be used for microbiological investigation during brewing and baking. Actidione (Cycloheximide) at a concentration of 0.001% permits the growth of bacteria and inhibits the growth of most yeasts and moulds except dermatophytes. This medium may be used for the estimation of bacterial contamination of pitching yeast. Addition of penicillin or streptomycin may be used for selective isolation of dermatophytes. HiVeg hydrolysate acts as source of nitrogen while yeast extract serves as a rich reservoir of vitamins. Dextrose in high amount along with mineral salts at acidic pH favour sugar fermentation.
Quality Control
Appearance of Powder
Light green coloured, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Greenish blue coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 7.53% w/v aqueous solution is pH 5.5 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 30°C for 40-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli (25922) | 102 | good-luxuriant | >50% |
| Lactobacillus fermentum (9338) | 102 | good-luxuriant | >50% |
| Proteus mirabilis (25933) | 102 | good-luxuriant | >50% |
| Saccharomyces cerevisiae (9763) | 102 | inhibited | 0% |
| Saccharomyces uvarum (9080) | 102 | inhibited | 0% |
References
- Green, S.R. and Gray, P.P., 1950, Wallerstein Lab. Communication, 13:357.
| Product Name | Actidione HiVeg™ Agar w/ Actidione® |
|---|---|
| SKU | MV400 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Green, S.R. and Gray, P.P. 1950, Wallerstein Lab. Communication 13,357. 2.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition 3.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 14.Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C. |
| Customized Product Available | No |
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Actidione Agar Base w/o Actidione®
As low as
$246.40
Actidione Agar w/ Actidione®
As low as
$778.80