Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Wilson Blair HiVeg™ Agar w/ BG
As low as
$46.20
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV332
For the isolation and preliminary identification of Salmonella Typhi from clinical specimens.
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Intended Use
Recommended for isolation and preliminary identification of Salmonella Typhi from clinical specimens.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiVeg™ extract | 5.000 |
| HiVeg™ peptone | 10.000 |
| Dextrose (Glucose) | 5.000 |
| Disodium hydrogen phosphate | 4.000 |
| Ferrous sulphate | 0.300 |
| Bismuth sulphite indicator | 8.000 |
| Brilliant green | 0.025 |
| Agar | 20.000 |
| Final pH (at 25°C) | 7.7±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 52.32 grams in 1000 ml purified/distilled water. Heat gently with frequent agitation until the medium is dissolved completely. DO NOT AUTOCLAVE. Cool to 50-55°C. Mix well to disperse precipitate and pour thick plates (25 ml medium per plate). Dry the plates before use, avoiding over drying.
Principle And Interpretation
Wilson and Blair Agar was formulated by Wilson and Blair (8) for isolating Salmonella species especially Salmonella serotype Typhi from clinical specimens. Wilson Blair HiVeg™ Agar w/ BG is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ extract and HiVeg™ peptone provide nitrogenous, carbonaceous compounds and other growth nutrients. Brilliant green dye inhibits all gram-positive bacteria. Dextrose is the fermentable carbohydrate. Ferrous sulphate is an indicator of H2S production. Bismuth is a heavy metal which is inhibitory to most gram-negative enteric bacilli other than Salmonella. Ferrous sulphate is reduced by Salmonella species in presence of bismuth sulphite and dextrose to form iron sulphide, indicated by black coloured colonies.
Type of specimen
Clinical samples - Faeces; Food and dairy samples; Water samples.
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,7,8). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(2) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Greenish yellow coloured homogeneous free flowing powder
Gelling
Firm, comparable with 2.0% agar gel.
Colour and Clarity of preapred medium
Light yellow coloured clear to slightly opalescent gel.
Reaction
Reaction of 5.23% w/v aqueous solution at 25°C. pH : 7.7±0.2
pH
7.50-7.90
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours
| Organism | Inoculum (CFU) |
Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | 0% | |
| Proteus mirabilis ATCC 25933 | 50-100 | luxuriant | >=50% | green |
| Salmonella Typhi ATCC 6539 | 50-100 | luxuriant | >=50% | black with sheen |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | >=50% | black with sheen |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Wilson Blair HiVeg™ Agar w/ BG |
|---|---|
| SKU | MV332 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Wilson and Blair, 1929, J. Pathol. Bacteriol., 29 : 310. 2.MacFaddin J., 1985, Media for the Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore. |
| Customized Product Available | No |
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