Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Soyabean HiVeg™ Medium w/ 0.1% Agar (Tryptone Soya HiVeg™ Broth w/ 0.1% Agar)
As low as
$225.50
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV323
For cultivation of anaerobes from root canals, blood and other specimens.
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Intended Use
Recommended for cultivation of anaerobes from various specimens.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Dipotassium hydrogen phosphate | 2.500 |
| Dextrose (Glucose) | 2.500 |
| Agar | 1.000 |
| Final pH (at 25°C) | 7.3±0.2 |
*Formula adjusted, standardized to suit performance parameters
Directions
Suspend 31.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Soyabean Casein Digest Medium with 0.1% Agar is used for culturing organisms especially anaerobes from root canals, blood and other clinical samples. Inclusion of agar to this medium is useful for isolating anaerobic oral Vibrio's (1) and also anaerobic organisms causing nasal sinusitis (2).
Soyabean HiVeg™ Medium w/ 0.1% Agar is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ hydrolysate and soya peptone supplies nitrogenous and carbonaceous compounds, trace minerals etc. Dextrose serves as a source of fermentable carbohydrate for the energy production. Sodium chloride maintains osmotic balance while Dipotassium phosphate provides buffering capacity. Small percentage of agar helps in creating moderately anaerobic condition in the depth of the medium.
Type of specimen
Please add specimens
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Biochemical characterization is necessary to be performed on colonies from pure cultures for further identification.
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured clear solution without any precipitate
Reaction: Reaction of 3.1% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH: 7.10-7.50
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 48 hours
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Bacteroides fragilis ATCC 25285 | 50-100 | good-luxuriant |
| Clostridium perfringens ATCC 12924 | 50-100 | good-luxuriant |
| Neisseria meningitidis ATCC 13090 | 50-100 | good |
| Staphylococcus epidermidis ATCC 12228 (00036*) | 50-100 | good-luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | good-luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
Key: * Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Soyabean HiVeg™ Medium w/ 0.1% Agar (Tryptone Soya HiVeg™ Broth w/ 0.1% Agar) |
|---|---|
| SKU | MV323 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Mashimo and Ellison, 1959, J. Bact., 78:636. 2.Fredette, Anger and Forget, 1961, Can. Med. Assoc. J., 84:164. |
| Customized Product Available | No |
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