Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Casein Soyabean Digest HiVeg™ Agar (Soyabean Casein Digest HiVeg™ Agar) (Tryptone Soya HiVeg™ Agar)
As low as
$36.30
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Availability: In stock
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SKU:
MV290
For cultivation of a wide variety of microorganisms from clinical and non-clinical samples and for sterility testing in pharmaceutical procedures.
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Intended use
Recommended as a general purpose medium used for cultivation of wide variety of microorganisms from various samples.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg® hydrolysate | 15.000 |
| Soya peptone | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, aseptically add 5% v/v defibrinated blood in previously cooled medium to 45-50°C for cultivation. Mix well and pour into sterile Petri plates.
Principle And Interpretation
This medium is prepared by completely replacing animal based peptones with vegetable peptones that are free of BSE/TSE risks. Soyabean HiVeg® Agar is the modification of Soyabean Casein Digest Agar with replacement of tryptone by HiVeg® hydrolysate. Soyabean HiVeg® Agar can be used as a general purpose medium used for multiple applications e.g. as a blood culture medium, as maintenance medium for culture collections (including maintenance of stock cultures), for testing bacterial contaminants and isolating fastidious organisms on enrichment with blood. It serves as a nutritive base to which variety of supplements can be added. On supplementation with blood it can be also used to determine haemolytic bacteria (1, 2). This medium can also be used for sensitivity testing by tube dilution method of antimicrobial agents, plate counting, against animal based Soyabean Casein Digest Agar.
This medium is employed for cultivation and isolation of fastidious and non-fastidious microorganisms. The combination of HiVeg® hydrolysate and soya peptone makes this media nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Sodium chloride maintains the osmotic balance.
Type of specimen
Pharmaceutical samples.
Specimen Collection and Handling:
For Pharmaceutical samples follow appropriate techniques for sample collection, handling and processing as per pharmacopeias (3,4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- 1. Further biochemical testing is required on pure colonies for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Basal Medium: Light yellow coloured clear to slightly opalescent gel. After addition of 5-7%w/v sterile defibrinated blood : Cherry red coloured opaque gel forms in Petri plates
pH of 4.0% w/v aqueous solution at 25°C .
pH
7.10-7.50
Cultural response
Cultural characteristics was observed after an incubation for Bacterial at 30-35°C 18-24 hours and for Fungal at 30-35°C <=5days.
| Organism | Inoculum (CFU) | Observed Lot value (CFU) | Recovery | Observed Lot value (CFU) w/blood | Recovery w/ blood | Haemolysis |
|---|---|---|---|---|---|---|
| ^Bacillus spizizenii ATCC 6633 (00003)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | none |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | beta |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | beta |
| Escherichia coli ATCC 25922 (00013)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | none |
| Escherichia coli ATCC 8739 (00012)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | none |
| Escherichia coli ATCC 11775 (00090)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | none |
| Escherichia coli NCTC 13167 (00179)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | none |
| Pseudomonas aeruginosa ATCC 27853 (00025)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| $Pseudomonas paraeruginosa ATCC 9027 (00026)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Pseudomonas aeruginosa ATCC 10145 (00024)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Salmonella Abony NCTC 6017(00029)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| @Kokuria rhizophila ATCC 9341 | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Salmonella Typhimurium ATCC 14028 (00031)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Enterococcus faecalis ATCC 29212 (00087)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Candida albicans ATCC 10231 (00054)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Candida albicans ATCC 2091 (00055)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| # Aspergillus brasiliensis ATCC 16404 (00053)* | 50-100 | 25-70 | 50-70% | |||
| Clostridium perfringenes ATCC 13124 (00007)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
Key: (#)- Formerly known as Aspergillus niger, (^)- Formerly known as Bacillus subtilis subsp. spizizenii, ($)- Formerly known as Pseudomonas aeruginosa, (@)- Formerly known as Micrococcus luteus(*) - Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Casein Soyabean Digest HiVeg™ Agar (Soyabean Casein Digest HiVeg™ Agar) (Tryptone Soya HiVeg™ Agar) |
|---|---|
| SKU | MV290 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. The United States Pharmacopoeia , 2018, The United States Pharmacopoeial Convention Inc., Rockville, MD. 2.Indian Pharmacopoeia, 2018, Govt. of India, Ministry of Health and Family Welfare, New Delhi, India. 3.Gunn B. A., Ohashi D K., Gaydos C. A., Holt E. S., 1977, J. Clin. Microbiol., 5(6) : 650. 4.Forbes B. A., Sahm A. S. and Weissfeld D. F., 1998, Bailey and Scotts Diagnostic Microbiology, 10th Ed., Mosby Inc.St. Louis, Mo5.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition. 6.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
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