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AATCC Bacteriostasis HiVeg™ Agar
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$240.90
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SKU:
MV231
Detection of antibacterial activity of fabrics.
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Intended Use
AATCC Bacteriostasis HiVeg Agar is used for the detection of antibacterial activity of fabrics while broth is used for routine antibacterial testing of antiseptics and disinfectants.
Detection of antibacterial activity of fabrics and antibacterial testing of antiseptics and disinfectants.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | |
|---|---|---|
| Product | MV231/MV221 | M231/M221 |
| Key Ingredients | HiVeg peptone HiVeg extract |
Peptic digest of animal tissue Beef extract |
Composition
| Ingredients | MV231 Grams/Litre | MV221 Grams/Litre |
|---|---|---|
| HiVeg peptone | 10.00 | 10.00 |
| HiVeg extract | 5.00 | 5.00 |
| Sodium chloride | 5.00 | 5.00 |
| Agar | 15.00 | - |
Final pH (at 25°C): MV231: 7.2 ± 0.2; MV221: 6.8 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 35 grams of MV231 or 20 grams of MV221 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
AATCC Bacteriostasis HiVeg Agar and AATCC Bacteriostasis HiVeg Broth are the modifications of AATCC Bacteriostasis Agar/Broth where the animal based peptones are substituted by vegetable peptones thus making the media BSE/TSE risk free. AATCC Bacteriostasis Agar is used in accordance with the standard procedure (1, 2, 3). AATCC Bacteriostasis Broth is useful for subcultures in phenol coefficient and dilution tests and tests of bacteriostatic, germicidal, sporicidal activity (1) and also as a base for the preparation of AATCC Bacteriostasis Agar (2). AATCC Bacteriostasis Agar may be used to carry stock cultures of Escherichia coli and Staphylococcus aureus. Also, it is used for the detection of antibacterial activity of fabrics. AATCC Bacteriostasis HiVeg Agar/Broth, like the conventional media serves the same above mentioned purposes.
The test cultures of Escherichia coli and Staphylococcus aureus are grown in AATCC Bacteriostasis HiVeg Broth for 24 hours. 1 ml of this culture is mixed with 150 ml of AATCC Bacteriostasis HiVeg Agar and poured into the plate. After the agar solidifies, apply a circular sterile test fabric of 28.6 mm diameter onto the plate. Incubate at 35-37°C for 18-24 hours and observe the inhibition of growth around test fabric.
HiVeg peptone and HiVeg extract provides nitrogenous nutrients and sodium chloride maintains the osmotic balance of the medium.
Quality Control
Appearance of Powder: Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel of MV231.
Colour and Clarity: Amber coloured, clear to slightly opalescent gel forms in petri plates, clear solution in tubes.
Reaction:
- Reaction of 3.5% w/v aqueous solution of MV231 is pH 7.2 ± 0.2 at 25°C.
- Reaction of 2.0% w/v aqueous solution of MV221 is pH 6.8 ± 0.2 at 25°C.
Technical Specifications
| Reconstitution | MV231: 35.0 g/l MV221: 20.0 g/l |
| Quantity on preparation (500g) | MV231: 14.28 L MV221: 25.0 L |
| pH (25°C) | MV231: 7.2 ± 0.2 MV221: 6.8 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium Below 30°C, Prepared Medium 2 - 8°C. |
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli (25922) | 102 | luxuriant | >70% |
| Staphylococcus aureus (6538) | 102 | luxuriant | >70% |
| Pseudomonas aeruginosa (27853) | 102 | luxuriant | >70% |
| Salmonella serotype Typhi (6539) | 102 | luxuriant | >70% |
Lack of bacterial growth indicates that the fabric has antibacterial activity.
References
- Williams (Ed.), 2005, Official methods of Analysis of AOAC, 18th ed. AOAC, Washington D.C.
- Tech. Manual of AATCC, 1985, Vol. 61, AATCC, Research Triangle Park, N.C.
- Ruuhle and Brewer, 1931, USFDA Methods of Testing Antiseptics and Disinfectants, USDA Circ.:198.
| Product Name | AATCC Bacteriostasis HiVeg™ Agar |
|---|---|
| SKU | MV231 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Williams (Ed.), 1995, Official methods of Analysis of AOAC, 16th ed. AOAC, Washington D.C.2 Tech. Manual of AATCC, 1985, Vol. 61, AATCC, Research Triangle Park,N.C.3 Ruuhle and Brewer, 1931, USFDA Methods of Testing Antiseptics and Disinfectants,USDA Circ.:198. |
| Customized Product Available | No |
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