Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Gifu Anaerobic HiVeg™ Broth Modified
As low as
$156.20
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV2079
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Recommended for the isolation and cultivation of anaerobic bacteria from clinical samples and susceptibility testing.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ peptone | 5.000 |
| Soya peptone | 3.000 |
| HiVeg™ peptone No.3 | 5.000 |
| Yeast extract | 12.50 |
| HiVeg™ extract | 3.400 |
| Dextrose (Glucose) | 0.500 |
| Potassium dihydrogen phosphate | 2.500 |
| Sodium chloride | 3.000 |
| Starch soluble | 5.000 |
| L-Cysteine hydrochloride | 0.300 |
| Sodium thioglycollate | 0.300 |
| L-Arginine | 1.000 |
| Vitamin K₁ | 0.005 |
| Iron salt | 0.010 |
| L-Tryptophan | 0.200 |
| Final pH (at 25°C) | 7.3±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 41.7 grams in 1000 ml of purified / distilled water. Heat if necessary to the dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 115°C for 15 minutes.
Principle And Interpretation
Gifu Anaerobic HiVeg™ Broth, Modified is a liquid medium for anaerobic bacteria. It is prepared by completely replacing animal based peptones by vegetable peptones to avoid BSE/TSE risks associated with animal peptones. As this medium contains combination of vegetable peptones, it is successfully used for cultivation of anaerobic organisms such as streptococci, pneumococci and meningococci. This medium is also suitable for blood culture (1). Anaerobic organisms require reducing condition and an absence of dissolved oxygen in the medium. Strict anaerobes obtain its energy and intermediates through oxidation utilizing hydrogen acceptors other than oxygen. Pre-reducing the medium by boiling to drive off the oxygen can expel this (2).
Sodium thioglycollate and L-Cysteine are the reducing agents added in this medium to provide adequate anaerobiosis. Anaerobic bacteria vary in their sensitivity to oxygen and nutritional requirements (3). HiVeg™ Peptone, HiVeg™ Peptone No.3, soya peptone, yeast extract and HiVeg™ extract provides nitrogen, carbon compounds, long chain amino acids, vitamin B complex and minerals for the growth of anaerobic organisms. Starch absorbs the toxic metabolites produced (4). Iron salt serves as essential growth factor and Sodium chloride maintains osmotic equilibrium (5).
Type of specimen
Clinical samples, Food samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (2,6).
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (7).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical testing is required for pure cultures for complete identification.
- This medium is recommended for susceptibility testing of pure cultures only.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to brownish yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Amber coloured clear solution (may have slight precipitate) forms in tube
Reaction
Reaction of 4.2% w/v aqueous solution at 25°C. pH : 7.3±0.2
pH
7.10-7.50
Cultural Response
Cultural characteristics observed in an anaerobic atmosphere after an incubation at 35 - 37°C for 48 - 72 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Streptococcus pyogenes ATCC 19615 | 50-100 | good - luxuriant |
| Phocaeicola vulgatus ATCC 8482 | 50-100 | good - luxuriant |
| Clostridium sporogens ATCC 11437 | 50-100 | good - luxuriant |
| Clostridium perfringens ATCC 13124 (00007*) | 50-100 | good - luxuriant |
Key: (*) Corresponding WDCM numbers.
** Formerly known as Bacteroides vulgatus
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,6).
| Product Name | Gifu Anaerobic HiVeg™ Broth Modified |
|---|---|
| SKU | MV2079 |
| Customized Product Available | No |
Write Your Own Review