Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Soyabean HiVeg™ Medium w/ Yeast Extract and Ferric pyrophosphate
As low as
$35.20
Availability: Out of stock
Availability: In stock
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SKU:
MV207
A highly nutritious medium which supports luxuriant growth of fastidious bacteria.
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Intended Use
Recommended for a highly nutritious medium which supports luxuriant growth of fastidious bacteria.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Dipotassium hydrogen phosphate | 2.500 |
| Yeast extract | 5.000 |
| Ferric pyrophosphate | 0.020 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 32.52 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Soyabean Casein Digest Medium with Yeast Extract and Hemin is prepared according to the formulation of USP (1). It is a highly nutritious medium for cultivating fastidious bacteria. It can also be used as general, all purpose cultivation medium (2,3).
Soyabean HiVeg™ Medium w/ Yeast Extract and Ferric pyrophosphate is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ hydrolysate, Soya peptone and yeast extract supply nitrogenous and carbonaceous nutrients, trace ingredients and vitamin B complex for the growth of microorganisms. Hemin provides additional growth factors. Dipotassium hydrogen phosphate maintains buffering conditions in the medium.
Type of specimen
Pharmaceutical samples.
Specimen Collection and Handling
For pharmaceutical samples, follow appropriate techniques for sample collection, processing as per pharmaceutical guidelines (1). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured clear solution without any precipitate
Reaction
Reaction of 3.25% w/v aqueous solution at 25°C. pH : 7.3±0.2
pH
7.10-7.50
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Bordetella pertussis ATCC 8467 | 50-100 | luxuriant |
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Soyabean HiVeg™ Medium w/ Yeast Extract and Ferric pyrophosphate |
|---|---|
| SKU | MV207 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. The United States Pharmacopeia, 1980, XX |
| Customized Product Available | No |
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