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Antibiotic HiVeg™ Assay Medium No. 37
As low as
$33.00
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1667
For cultivation of a wide variety of microorganisms and sterility testing of pharmaceutical preparations.
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Composition**
| Ingredients | Gms/Litre |
|---|---|
| HiVeg hydrolysate | 17.000 |
| Papaic digest of soyabean meal | 3.000 |
| Dextrose | 2.500 |
| Sodium chloride | 5.000 |
| Dipotassium phosphate | 2.500 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30 grams in 1000 ml purified/distilled water. Heat to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 25°C and store in a cool dark place preferably below 25°C.
Principle And Interpretation
Antibiotic HiVeg Assay Medium No. 37 is prepared by incorporating vegetable peptones in place of animal peptones, making the medium BSE-TSE risks free. Grove and Randall have elucidated the antibiotic assays and media in their comprehensive treatise on antibiotic assays (1) and are also formulated as per CFR and USP (2,3).
Antibiotic HiVeg Assay Medium No. 37 can be used for the same purpose of Antibiotic Assay Medium No. 37. Hence Antibiotic HiVeg Assay Medium No. 37 can also be used for cultivation of fastidious and non-fastidious organisms and sterility testing of pharmaceutical preparations.
It is also used for the sensitivity testing by the tube dilution method for antimicrobial agents (4). Turbidimetric antibiotic assay is based on the change or inhibition of growth of a test microorganisms in a liquid medium containing a uniform concentration of an antibiotic. After incubation of the test organism in the working dilutions of the antibiotics, the amount of growth is determined by measuring the light transmittance using spectrophotometer. The concentration of antibiotic is determined by comparing amounts of growth obtained with that given by the reference standard solutions. Use of this method is appropriate only when test samples are clear.
The combination of HiVeg hydrolysate and Papaic digest of soyabean meal makes this medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Dextrose serves as the carbohydrate source and dipotassium phosphate faclitates buffering in the medium. Sodium chloride maintains the osmotic balance of the medium.
Note: For Antibiotic Assay Methods and Selection of Antibiotic HiVeg Assay Medias Refer Section Antibiotic HiVeg Assay Media.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured clear solution without any precipitate in tubes.
Reaction: Reaction of 3.0% w/v aqueous solution at 25°C. pH : 7.3±0.2
pH: 7.10-7.50
Cultural Response
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Growth at 30-35°C for <= 3 days | ||
| Escherichia coli ATCC 25922 | 50-100 | luxuriant |
| Escherichia coli ATCC 8739 | 50-100 | luxuriant |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant |
| Salmonella Ebony NCTC 6017 | 50-100 | luxuriant |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant |
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant |
| Staphylococcus aureus ATCC25923 | 50-100 | luxuriant |
| Staphylococcus aureus ATCC 6538 | 50-100 | luxuriant |
| Micrococcus luteus ATCC 9341 | 50-100 | luxuriant |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant |
| Growth at 20-25°C for <= 5 days | ||
| Candida albicans ATCC10231 | 50-100 | luxuriant |
| Candida albicans ATCC 2091 | 50-100 | luxuriant |
| *Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on label.
| Product Name | Antibiotic HiVeg™ Assay Medium No. 37 |
|---|---|
| SKU | MV1667 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Grove and Randall, 1955, Assay Methods of Antibiotics, Medical Encyclopedia, Inc. New York |
| Customized Product Available | No |
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