Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Leifson HiVeg™ Agar
As low as
$273.90
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1380
For isolation of Salmonella and Shigella species.
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Intended use
Recommended for isolation of Salmonella and Shigella species.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ extract No. 1 | 5.000 |
| HiVeg™ peptone No. 1 | 5.000 |
| Lactose | 10.000 |
| Sodium thiosulphate | 5.400 |
| Sodium citrate | 6.000 |
| Ferric citrate | 1.000 |
| Synthetic detergent No. III | 3.000 |
| Neutral red | 0.020 |
| Agar | 12.000 |
Final pH (at 25°C) 7.5±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 47.42 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE OR OVERHEAT. Excessive heating is detrimental.Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Salmonella and Shigella are gram-negative, facultatively anaerobic, non-sporulating, non-motile rods in the family Enterobacteriaceae. They are widely distributed in animals affecting mainly the stomach and the intestines. Leifson Agar is recommended for isolation of Salmonella and Shigella species (1). Leifson HiVeg™ Agar is same as Leifson Agar except that the animal based peptones are completely replaced with vegetable peptones to avoid the BSE/TSE risks associated with animal peptones. HiVeg™ extract No. 1 and HiVeg™ peptone No. 1 provides nitrogenous and carbonaceous compounds, long chain amino acids and other essential growth nutrients. Synthetic detergent No. III inhibit all gram-positive bacteria. Lactose is added to the medium to allow differentiation of lactose fermenting bacteria such as Escherichia coli from non-lactose fermenting species such as Salmonella and Shigella species. Lactose fermenting strains grow as red to pink colonies because of absorption of neutral red indicator. Sodium thiosulphate and ferric citrate forms the H2S indicator system. Non-fermenting species grow as colorless colonies with black centers due to production of H2S against Shigella which does not produce H2S (2).
Type of specimen
Food and dairy samples; Water samples.
Specimen Collection and Handling:
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3,4,5). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(4) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm, comparable with 1.2% Agar gel.
Colour and Clarity of prepared medium
Reddish orange coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.74% w/v aqueous solution at 25°C. pH: 7.5±0.2
pH
7.30-7.70
Cultural Response
MV1380: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony | H2S |
|---|---|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | poor | 10-20% | pink w/bile precipitate | negative reaction |
| Enterococcus faecalis ATCC 29212 (00087*) | >=104 | inhibited | 0% | ||
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | good-luxuriant | >=50% | colourless | positive reaction, black centred colonies |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | good | 40-50% | colourless | negative reaction |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | >=50% | colourless | positive reaction, black centred colonies |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Leifson HiVeg™ Agar |
|---|---|
| SKU | MV1380 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Leifson, E., 1935, J. Pathol. Bacteriol., 40-581. |
| Customized Product Available | No |
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