Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Tryptophan HiVeg™ Medium
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$176.00
Availability: Out of stock
Availability: In stock
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SKU:
MV1339
for detection of indole production.
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Intended Use
Tryptophan HiVeg Medium is used for detection of indole reaction production.
Recommended for detection of indole reaction production.
Product Variants
Vegetable based (Code MV)
Code: MV1339
Composition detail: HiVeg hydrolysate
Animal based (Code M)
Code: M1339
Composition detail: Casein enzymic hydrolysate
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 10.00 |
| Sodium chloride | 5.00 |
| DL-Tryptophan | 1.00 |
| Final pH (at 25°C) | 7.5 ± 0.2 |
** Formula adjusted, standardized to suit performance parameters.
Reconstitution: 16.0 g/l
Quantity on preparation (500g): 31.25 L
Supplement: None
Directions
Suspend 16 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Sterilization: 121°C / 15 minutes.
Principle and Interpretation
This medium is prepared by using HiVeg hydrolysate which is free of BSE/TSE risks. This medium is the modification of Tryptophan Medium which is a modification of original formula of APHA where the medium is devoid of Tryptophan (1). This medium is useful for the detection of indole production which is a key feature in the differentiation of coliforms. HiVeg hydrolysate provides carbonaceous and nitrogenous sources required for the growth of microorganisms. Tryptophan is an amino acid, which is broken by certain microorganisms with the help of enzymes. The various enzymes involved are collectively called as tryptophanase, a general term used o denote the complete system of enzymes that mediate the indole production by hydrolytic activity on tryptophan (2). The indole produced can be detected by either Kovac's or Ehrlich's reagent (3). Indole combines with the aldehyde present in the above reagent to give red colour in the alcohol layer. The alcohol layer extracts and concentrates the red colour complex.
Quality Control
Appearance of powder: Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder
Colour and Clarity: Yellow coloured, clear solution without any precipitate
Reaction: Reaction of 1.6% w/v aqueous solution is pH 7.5 ± 0.2 at 25°C
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24 hours.
| Organisms (ATCC) | Indole reaction |
|---|---|
| Enterobacter aerogenes (13048) | - |
| Escherichia coli O157:H7 | + |
| Escherichia coli (25922) | + |
Storage and Shelf Life
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
References
- Greenberg A.E., Clesceri L.S. and Eaton A.D. (Eds.), 1992, Standard methods for the Examination of Water and Wastewater, 18th ed. APHA., Washington, D.C.
- MacFaddin, 1980, Biochemical Tests for Identification of Medical Bacteria, 2nd ed. Williams and Wilkins Baltimore.
- Finegold and Baron, 1986, Bailey and Scott's Diagnostic Microbiology, 7th ed., The C.V. Mosby Co., St. Louis.
| Product Name | Tryptophan HiVeg™ Medium |
|---|---|
| SKU | MV1339 |
| Customized Product Available | No |
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