Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Lecithin HiVeg™ Agar
As low as
$258.50
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1325
Recommended for detection of bacterial contamination of surfaces in unprotected and protected areas.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Lecithin HiVeg Agar is recommended for detection of bacterial contamination of surfaces in protected and unprotected areas.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 15.0 |
| Papaic digest of soyabean meal | 5.0 |
| Sodium chloride | 5.0 |
| Lecithin | 0.7 |
| Polysorbate 80 | 5.0 |
| Sodium thiosulphate | 1.0 |
| L-Histidine | 1.0 |
| Agar | 20.5 |
Final pH (at 25°C) 7.3 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 53.2 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Lecithin HiVeg Agar is prepared by using HiVeg hydrolysate which is free of BSE/TSE risks associated with animal based peptones. This medium is the modification of the medium which was originally recommended by APHA for use in microbial testing of water (1). Lecithin and polysorbate 80 is also added in this medium, as in the conventional medium by Weber and Black as a result of their research of the relative efficiencies of inhibitors for quaternary ammonium compounds (2). This medium is recommended for screening cosmetic products for microbial contamination.
In Lecithin HiVeg Agar, Papaic digest of soyabean meal and HiVeg hydrolysate provide nitrogenous compounds, carbon, sulphur and trace ingredients. Lecithin neutralizes quaternary ammonium compounds and polysorbate 80 is added to nullify phenolic compounds, hexachlorophene, formalin and along with lecithin neutralizes ethyl alcohol (3).
Recommended for
Detection of bacterial contamination of surfaces in protected and unprotected areas.
Reconstitution
53.2 g/l
Quantity on preparation (500g)
9.39 L
pH (25°C)
7.3 ± 0.2
Supplement
None
Sterilization
121°C / 15 minutes
Storage
Dry Medium and Prepared Medium 2 - 8°C.
Quality Control
Appearance of powder
Yellow coloured may have slightly greenish tinge homogeneous, free flowing powder
Gelling
Firm, comparable with 2.05% Agar gel.
Colour and Clarity
Yellow coloured, slightly opalescent gel forms in petri plates.
Reaction
Reaction of 5.32% w/v auqeous solution is pH 7.3 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli (25922) | 10²-10³ | luxuriant | >70% |
| Staphylococcus aureus (25923) | 10²-10³ | luxuriant | >70% |
References
- Eaton A.D., Clesceri L.S. and Greenberg A.E., (Eds.), 2005, Standard Methods for the Examination of Water and Wastewater, 21st ed, APHA, Washington, D.C.
- Weber and Black, 1948, Soap Sanitary Chem., 24:134.
- Favero (Chm.), 1967, A State of the Art Report, Biological Contamination Control Committee, American Association for Contamination Control.
| Product Name | Lecithin HiVeg™ Agar |
|---|---|
| SKU | MV1325 |
| Customized Product Available | No |
Write Your Own Review