Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Dextrose Tryptone HiVeg™ Broth
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$162.80
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SKU:
MV122
For enrichment and cultivation of mesophilic and thermophilic organisms in food.
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Dextrose Tryptone HiVeg Agar / Broth media are recommended for the detection and enumeration of mesophilic and thermophilic aerobic microorganisms in foods.
Composition
| Ingredients | MV092 Grams/Litre |
MV122 Grams/Litre |
MV913 Grams/Litre |
MV914 Grams/Litre |
|---|---|---|---|---|
| HiVeg hydrolysate | 10.00 | 10.00 | 10.00 | 10.00 |
| Dextrose | 5.00 | 5.00 | 5.00 | 5.00 |
| Dipotassium phosphate | — | — | 1.25 | 1.25 |
| Yeast extract | — | — | 1.00 | 1.00 |
| Bromo cresol purple | 0.04 | 0.04 | 0.04 | 0.04 |
| Agar | 15.00 | — | 15.00 | — |
Final pH (at 25°C) 6.7 ± 0.2
** Formula adjusted, standardized to suit performance parameters
Product Profile
Vegetable based (Code MV)©
MV092/MV122/MV913/MV914
HiVeg hydrolysate
Animal based (Code M)
M092/M122/M913/M914
Casein enzymic hydrolysate
Recommended for
Detection and enumeration of mesophilic and thermophilic aerobic microorganisms in foods.
Reconstitution
(MV092): 30.0 g/l
(MV122): 15.0 g/l
(MV913): 32.3 g/l
(MV914): 17.3 g/l
Quantity on preparation (500g)
(MV092): 16.66 L
(MV122): 33.33 L
(MV913): 15.47 L
(MV914): 28.90 L
pH (25°C)
6.7 ± 0.2
Supplement
None
Sterilization
121°C / 15 minutes.
Storage
Dry Medium - Below 30°C, Prepared Medium 2-8°C.
Directions
Suspend 30 grams of MV092 or 15 grams of MV122 or 32.3 grams of MV913 or 17.3 grams of MV914 in 1000 ml distilled water. Heat to boiling the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Dextrose Tryptone HiVeg Media are prepared by using HiVeg hydrolysate in place of Casein enzymic hydrolysate which makes the media free of BSE/TSE risks. Dextrose Tryptone HiVeg Agar is the modification of the medium evolved by Williams (1) for cultivation and enumeration of the thermophilic bacteria. Its use for routine culture purpose is recommended by Cameron (2) and the Association of Official Agricultural Chemists (3). Dextrose Tryptone HiVeg Agar / Broth, Modified (MV913 / MV914) are more nutritious and well buffered than Dextrose Tryptone HiVeg Agar / Broth (MV092 / MV122) due to inclusion of yeast extract and dipotassium phosphate. Dextrose Tryptone HiVeg Agar/ Broth, Modified is used for the examination of canned food, sugar and starch for thermophilic bacteria such as Bacillus stearothermophillus ('flat sour'spoilage bacteria) (4) and also for plate count of mesophilic or thermophilic aerobes in sweetening agents used in frozen desserts (5) and for counts of aerobic microorganisms in liquid sugar. HiVeg hydrolysate provides nutrients to the organisms. Dextrose serves as an energy source while bromo cresol purple is a pH indicator. Acid producing organisms produce yellow coloured colony. The plates / tubes should be incubated at 55°C for 48 hours in a humid incubator. These media are useful for enumeration of mesophilic organisms, thermophiles in cereals and cereal products, dehydrated fruits vegetables and spices (6)
Colour and Clarity
Purple coloured, slightly opalescent gel forms in petri plates, clear solution in tubes.
Reaction
Reaction of 3.0 w/v of MV092 or 1.5% w/v of MV122 or 3.23% w/v of MV913 or 1.73% w/v of MV914 aqueous solution is pH 6.7 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 55°C for 48 hours.
| Organisms (ATCC) | Inoculum (CFU) |
Growth | Recovery | Colour of colony on the Agar Media |
|---|---|---|---|---|
| Bacillus coagulans (8038) |
102-103 | good-luxuriant | > 70% | yellow |
| Bacillus stearothermophilus (7953) |
102-103 | good-luxuriant | > 70% | yellow |
| Bacillus brevis (8246) | 102-103 | good-luxuriant* | > 70% | yellow |
Key : * = with or without dextrose fermentation
Quality Control
Appearance of Powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel of MV092 and MV913.
| Product Name | Dextrose Tryptone HiVeg™ Broth |
|---|---|
| SKU | MV122 |
| Customized Product Available | No |
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