Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Coliform HiVeg™ Broth
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$232.10
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SKU:
MV1211
For isolation and cultivation of coliform organisms from cream, yogurt and raw milk.
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Intended Use
Coliform HiVeg Broth is recommended for isolation and cultivation of coliform organisms from cream, yogurt and raw milk.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone No. 3 | 15.0 |
| Yeast extract | 6.0 |
| Synthetic detergent | 5.0 |
| Synthetic detergent No. III | 0.1 |
| Lactose | 20.0 |
| Sodium lauryl sulphate | 1.0 |
| Bromo cresol purple | 0.035 |
| HiVeg peptone | 10.0 |
| Final pH (at 25°C) | 7.0 ± 0.2 |
** Formula adjusted, standardized to suit performance parameters.
Product Profile
Vegetable based (Code MV)
MV1211
- HiVeg peptone No. 3
- Synthetic detergent
- Synthetic detergent No. III
- HiVeg peptone
Animal based (Code M)
M1211
- Proteose peptone
- Bile salts
- Sodium deoxycholate
- Peptic digest of animal tissue
Recommended for
Isolation and cultivation of coliforms from cream, yogurt and raw milk.
Directions
Suspend 57.14 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Technical Data
| Reconstitution | 57.14 g/l |
| Quantity on preparation (500g) | 8.75 L |
| pH (25°C) | 7.0 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
Storage: Dry Medium Below 30°C, Prepared Medium 2 - 8°C.
Principle and Interpretation
This medium is prepared by completely replacing animal based peptones with vegetable peptones which are free from BSE/TSE risks. Coliform HiVeg Broth is the modification of Coliform Broth which is recommended for isolation and cultivation of coliforms from cream, yogurt and raw milk (1) HiVeg peptone No. 3, HiVeg peptone and yeast extract provides nitrogeneous carbonaceous compounds and vitamin B complex for the growth of coliform. Lactose is the fermentable carbohydrate. Bromocreso purple is the pH indicator. Coliforms ferment lactose and produce acid and gas, which is indicated by change in colour of medium to yellow. Synthetic detergents inhibit gram positive bacteria. Sodium lauryl sulphate is inhibitory to many organisms but not to coliforms.
Quality Control
Appearance of powder: Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity: Purple coloured, clear solution without any precipitate.
Reaction: Reaction of 5.71% w/v aqueous solution is pH 7.0 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 48 hours.
Organisms (ATCC)
| Organisms (ATCC) | Inoculum (CFU) | Growth | Acid | Gas |
|---|---|---|---|---|
| Enterobacter aerogenes (13048) | 102-103 | good-luxuriant | + | + |
| Escherichia coli (25922) | 102-103 | good-luxuriant | + | + |
| Staphylococcus aureus (25923) | 102-103 | inhibited | - | - |
KEY : Acid
- + : Positive reaction, yellow colour
- - : Negative reaction purple or no colour change
Visual Results
MV1211 Coliform HiVeg Broth
- Control
- Escherichia coli
- Enterobacter aerogenes
- Staphylococcus aureus
References
- Vanderzant C and Splittstoesser DF (Ed), 1992, Compendium of Methods for the Microbiological Examination of Foods 3rd ed, APHA Washington, DC.
| Product Name | Coliform HiVeg™ Broth |
|---|---|
| SKU | MV1211 |
| Customized Product Available | No |
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