Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Fluid Lactose HiVeg™ Medium w/ Soya Lecithin and Polysorbate 20 (Twin Pack)
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$80.30
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SKU:
MV1188
For microbial evaluation of oral hygiene products.
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Intended Use
Fluid Lactose HiVeg Medium with Soya Lecithin & Polysorbate 20 is recommended for microbial evaluation of oral hygiene products.
Product Profile
| Category | Vegetable based (Code MV) | Animal based (Code M) |
|---|---|---|
| Code | MV1188 | M1188 |
| Extract | HiVeg extract | Beef extract |
| Peptone | HiVeg peptone No.2 | Pancreatic digest of gelatin |
| Recommended for | Microbial evaluation of oral hygiene products. |
| Reconstitution | 18.0 g/l (Part A) + 40.0 ml/l (Part B) |
| Quantity on preparation (500g) | 8.62 L (A+B) |
| (100g) | 1.72 L (A+B) |
| pH (25°C) | 6.9 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium and Prepared Medium 2-8°C. |
Composition
| Ingredients | Grams/Litre |
|---|---|
| Part A: | |
| HiVeg extract | 3.00 |
| HiVeg peptone No. 2 | 5.00 |
| Lactose | 5.00 |
| Soya lecithin | 5.00 |
| Part B: | |
| Polysorbate 20 | 40 ml |
Final pH (at 25°C) 6.9 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 18.0 grams of Part A in 960 ml distilled water. Heat if necessary to dissolve the medium completely. Add 40 ml of Part B. Mix well and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by using HiVeg peptone No.2 and HiVeg extract which makes the medium free from BSE/TSE risks. Fluid Lactose HiVeg Medium with Soya Lecithin and polysorbate 20 like the conventional medium is recommended for microbial evaluation of oral hygiene products.
HiVeg extract and HiVeg peptone No.2 provide essential nutrients for bacterial metabolism. Lactose serves as a source of carbon. Soya lecithin and polysorbate 20, two commonly used neutralizers are reported to inactivate residual disinfectants when the sample is being collected. Soya lecithin neutralizes the quaternary ammonium compounds while polysorbate 20 neutralizes phenolic disinfectants; hexachlorophene and formalin (1).
Quality Control
Appearance of powder
- Part A : Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder
- Part B : Colourless, viscous solution.
Colour and Clarity
Yellow coloured, clear to slightly opalescent solution
Reaction
Reaction of the medium (1.8% w/v Part A + 4.0% v/v Part B) is pH 6.9 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| *Candida albicans (26790) | 102-103 | luxuriant |
| Enterococcus faecalis (29212) | 102-103 | luxuriant |
| Escherichia coli (25922) | 102-103 | luxuriant |
| Pseudomonas aeruginosa (27853) | 102-103 | luxuriant |
| Staphylococcus aureus (25923) | 102-103 | luxuriant |
key: * = incubate at 25-30°C upto 72 hours.
References
- Faverco [Chem.], 1967, Microbiological Sampling of Surfaces, Biological Contamination Control Committee, American Assoc. for Contamination Control
- Murray P. R, Baren E. J, Jorgensen J. H, Pfaller M. A, Yolken R. H (eds) 2003, Manual of clinical Microbiology, 8th ed., ASM, Washington, D.C.
| Product Name | Fluid Lactose HiVeg™ Medium w/ Soya Lecithin and Polysorbate 20 (Twin Pack) |
|---|---|
| SKU | MV1188 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Faverco [chem.], 1967, Microbiological Sampling of Surfaces, Biological Contamination Control Committee, AmericanAssoc. for Contamination Control |
| Customized Product Available | No |
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