Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Lactobacillus Selection HiVeg™ Agar Base
As low as
$240.90
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Availability: In stock
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SKU:
MV1180
For isolation and enumeration of Lactobacilli from food.
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Intended Use
Lactobacillus Selection HiVeg Agar / Broth Base is recommended for isolation and enumeration of Lactobacilli from foods.
Product Specifications
Product Profile:
Vegetable based (Code MV): MV1180/MV1166, HiVeg hydrolysate
Animal based (Code M): M1180/M1166, Casein enzymic hydrolysate
Recommended for: Isolation and enumeration of Lactobacilli from foods.
Reconstitution:
- (MV1180): 84.7 g/l
- (MV1166): 69.7 g/l
Quantity on preparation (500g):
- (MV1180): 5.90 L
- (MV1166): 7.17 L
pH (25°C):
- (MV1180): 5.5 ± 0.2
- (MV1166): 5.4 ± 0.2
Supplement: Glacial acetic acid
Sterilization: Boiling (DO NOT AUTOCLAVE) / 118°C / 15 minutes (if medium is to be stored).
Storage: Dry Medium and Prepared Medium 2 - 8°C.
Reaction of 6.97% w/v aqueous solution of MV1166 is pH 5.4 ± 0.2 at 25°C.
Composition**
| Ingredients | MV1180 Grams/Litre |
MV1166 Grams/Litre |
|---|---|---|
| HiVeg hydrolysate | 10.00 | 10.00 |
| Yeast extract | 5.00 | 5.00 |
| Dextrose | 20.00 | 20.00 |
| Sodium acetate | 25.00 | 25.00 |
| Monopotassium hydrogen phosphate | 6.00 | 6.00 |
| Ammonium citrate | 2.00 | 2.00 |
| Polysorbate 80 | 1.00 | 1.00 |
| Magnesium sulphate | 0.575 | 0.575 |
| Manganese sulphate | 0.12 | 0.12 |
| Ferrous sulphate | 0.034 | 0.034 |
| Agar | 15.00 | — |
Final pH (at 25°C): 5.5 ± 0.2 (MV1180), 5.4 ± 0.2 (MV1166)
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 84.7 grams of MV1180 or 69.7 grams of MV1166 in 1000 ml distilled water containing 1.32 ml glacial acetic acid. Heat with frequent stirring. Boil for 1 - 2 minutes to dissolve the medium completely. DO NOT AUTOCLAVE. If storage is necessary, autoclave at 12 lbs pressure (118°C) for 15 minutes.
Principle and Interpretation
These media are prepared by using HiVeg hydrolysate in place of Casein enzymic hydrolysate which make the medium free of BSE/TSE risks. Lactobacillus Selection HiVeg Agar Base is used for isolation and enumeration of Lactobacilli. Lactobacillus Selection HiVeg Broth Base may be used for isolation and cultivation of Lactobacilli. These media are the modifications of the media developed by Rogosa et al (1, 2) as a selective media for isolation and enumeration of Lactobacilli from oral, faecal specimens (3), food (4) and dairy products (5).
HiVeg hydrolysate, yeast extract and dextrose are the nitrogen and carbon sources. Polysorbate 80 provides fatty acids required for the metabolism of Lactobacilli. Ammonium citrate and sodium acetate inhibit many organisms, including Streptococci, moulds and also restrict swarming. Addition of acetic acid lowers the pH which is inhibitory to many microorganisms but favours the growth of Lactobacilli on the agar medium. Lactobacilli appear as large, white colonies.
Quality Control
Appearance of Powder: Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel of MV1180.
Colour and Clarity: Yellow coloured, slightly opalescent gel forms in petri plates, clear to slightly opalescent solution in tubes.
Reaction: Reaction of 8.47% w/v aqueous solution of MV1180 is pH 5.5 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 48 hours, in presence of 3 - 5% CO2.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Lactobacillus acidophilus (4356) | 102-103 | luxuriant | >70% |
| Lactobacillus plantarum (8014) | 102-103 | luxuriant | >70% |
| Lactobacillus casei (9595) | 102-103 | luxuriant | >70% |
| Enterococcus faecalis (29212) | 102-103 | inhibited | 0% |
| Proteus vulgaris (13315) | 102-103 | inhibited | 0% |
References
- Rogosa, Mitchell and Wiseman, 1951, J. Bacteriol., 62:132.
- Rogosa, Mitchell and Wiseman, 1951, J. Dental Res., 30:682.
- Ellis and Sarles, 1958, J. Bacteriol., 75:272.
- Downes FP and Ito K (Eds.), 2001, Compendium of Methods For The Microbiological Examination of Foods, 4th ed., APHA, Washington, D.C.
- Standard Methods for the Examination of Dairy Products, 17th Edition, 2004 Edited by H. Michael Wehr and Joseph H.Frank.
| Product Name | Lactobacillus Selection HiVeg™ Agar Base |
|---|---|
| SKU | MV1180 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Rogosa, Mitchell and Wiseman, 1951, J. Bacteriol., 62:132.2.Rogosa, Mitchell and Wiseman, 1951, J. Dental Res., 30:682.3.Ellis and Sarles, 1958, J. Bacteriol., 75:272.4.Speck M. (Ed.), 1984, Compendium of Methods for the Microbiological Examination of Foods, 2nd ed., APHA, Washington,D.C. |
| Customized Product Available | No |
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