Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Pre - Enrichment HiVeg™ Broth Base
As low as
$225.50
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SKU:
MV1178
For isolation and enrichment of Yersinia enterocolitica from food.
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Intended Use
Pre-Enrichment HiVeg Broth Base is used for isolation and enrichment of Yersinia enterocolitica from foods.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg special peptone | 10.0 |
| Yeast extract | 20.0 |
| Disodium phosphate | 7.1 |
| Sodium chloride | 1.0 |
| Potassium chloride | 1.0 |
Final pH (at 25°C) 8.3 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Product Technical Data
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1178 | M1178 |
| HiVeg special peptone | Peptone special |
- Recommended for: Isolation and enrichment of Yersinia enterocolitica from foods
- Reconstitution: 39.1 g/l
- Quantity on preparation (500g): 12.78 L
- pH (25°C): 8.3 ± 0.2
- Supplement: Filter sterilized magnesium sulphate (0.1%) and calcium chloride (0.1%) solution
- Sterilization: 121°C / 15 minutes.
- Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 39.1 grams in 980 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add filter sterilized magnesium sulphate (0.1%) and calcium chloride (0.1%) solution. Mix well before dispensing into sterile tubes.
Principle and Interpretation
Pre-Enrichment HiVeg Broth is prepared by using HiVeg special peptone making the medium free of BSE/TSE risks. Pre - Enrichment HiVeg Broth is the modification of Pre - Enrichment Broth which is formulated as recommended by APHA (1) for isolation and enrichment of Yersinia enterocolitica, a foodborne pathogen responsible for gastroenteritis (2).
Yeast extract and HiVeg special peptone supply essential nutrients like vitamin B complex, nitrogen compounds and trace ingredients. Sodium chloride and disodium phosphate protects the medium against any osmotic and pH imbalance respectively. Magnesium sulphate, calcium chloride and potassium chloride provide the ions necessary for the growth of the organisms.
Prepare 1:10 homogenate of food sample by weighing 25 gm of food and adding it to Pre - Enrichment HiVeg Broth. Prepare a homogenate by blending or in a stomacher and incubate at 10°C for 3 days. Inoculate this pre-enriched culture in PSB HiVeg Broth Base (MV941) and incubate at 25°C and streak onto a plating agar as MacConkey HiVeg Agar (MV081) or SS HiVeg Agar (MV108) after 3 and 5 days.
Quality Control
Appearance of powder
Light yellow coloured may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Yellow coloured, clear solution with slight precipitate.
Reaction
Reaction of 3.91% w/v aqueous solution is pH 8.3 ± 0.2 at 25°C
Cultural Response
Cultural characteristics observed after an incubation at 30 - 32°C for 18 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Yersinia enterocolitica (27729) | 102-103 | luxuriant |
| Product Name | Pre - Enrichment HiVeg™ Broth Base |
|---|---|
| SKU | MV1178 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Vanderzant C. and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination ofFoods, 3rd Ed., APHA, Washington, D.C. |
| Customized Product Available | No |
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Pre - Enrichment Broth Base
As low as
$224.40
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