Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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PL HiVeg™ Agar
As low as
$349.80
Availability: Out of stock
Availability: In stock
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SKU:
MV1173
For the isolation and cultivation of Plesiomonas shigelloides from food.
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Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1173 | M1173 |
| HiVeg peptone | Peptic digest of animal tissue |
| Synthetic detergent | Bile salts |
Recommended for: Isolation and cultivation of Plesiomonas shigelloides from food.
Reconstitution: 43.58 g/l
Quantity on preparation (500g): 11.47 L
pH (25°C): 7.4 ± 0.2
Supplement: None
Sterilization: 115°C / 15 minutes
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 5.0 |
| Yeast extract | 2.0 |
| Sodium chloride | 5.0 |
| Mannitol | 7.5 |
| L-Arabinose | 5.0 |
| Inositol | 1.0 |
| Lysine | 2.0 |
| Synthetic detergent | 1.0 |
| Phenol red | 0.08 |
| Agar | 15.0 |
| Final pH (at 25°C) | 7.4 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 43.58 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes.
Principle and Interpretation
PL HiVeg Agar is prepared by using HiVeg peptone and synthetic detergent which are free of BSE/TSE risks associated with animal based peptones. PL HiVeg Agar is the modification of PL Agar which is formulated as per APHA (1) for isolation and cultivation of Plesiomonas shigelloides from foods. Plesiomonas shigelloides is an opportunistic pathogen while controversy exists as its role as an enteropathogen (2, 3) and is mainly associated with the consumption of uncooked molluscs or with foreign travel (4). It does not grow on media like Thiosulphate Citrate Salts Sucrose HiVeg Agar (TCBS HiVeg Agar, MV189) but grows well on PL HiVeg Agar.
HiVeg peptone and yeast extract supply the nitrogenous compounds, vitamin B complex and trace ingredients. L-lysine is the amino acid source while arabinose, inositol and mannitol are the fermentable carbohydrate sources in the medium. Synthetic detergent inhibits gram-positive bacteria. Phenol red is the pH indicator which turns yellow at acidic pH.
If the sample is fluid, streak directly. If the sample is solid, prepare dilution in 0.1% HiVeg Peptone Water, (MV028) or transfer 10 gram sample to 90 ml Tetrathionate HiVeg Broth (MV032) without iodine and incubate at 40°C for 24 hours. After incubation streak on PL HiVeg Agar.
Quality Control
Appearance of powder: Pink coloured, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity: Red coloured, clear to slightly opalescent gel forms in petri plates.
Reaction: Reaction of 4.36% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Plesiomonas shigelloides (14029) | 102-103 | luxuriant |
| Product Name | PL HiVeg™ Agar |
|---|---|
| SKU | MV1173 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Ingram C. W., Morrison A. J. and Levitz R. E., 1987, J. Clin. Microbiol., 25 : 1791. |
| Customized Product Available | No |
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PL Agar
As low as
$349.80