Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Lactobacillus Selection HiVeg™ Broth Base
As low as
$256.30
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1166
For the selective isolation, cultivation and enumeration of Lactobacilli from food.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Lactobacillus Selection HiVeg Agar / Broth Base is recommended for isolation and enumeration of Lactobacilli from foods.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | |
|---|---|---|
| Product Type | MV1180/MV1166 | M1180/M1166 |
| Base Ingredient | HiVeg hydrolysate | Casein enzymic hydrolysate |
| Reconstitution (MV1180) | : 84.7 g/l | |
| Reconstitution (MV1166) | : 69.7 g/l | |
| Quantity on preparation (500g) (MV1180) | : 5.90 L | |
| Quantity on preparation (500g) (MV1166) | : 7.17 L | |
| pH (25°C) (MV1180) | : 5.5 ± 0.2 | |
| pH (25°C) (MV1166) | : 5.4 ± 0.2 | |
| Supplement | : Glacial acetic acid | |
| Sterilization | : Boiling (DO NOT AUTOCLAVE) / 118°C / 15 minutes (if medium is to be stored). | |
| Storage | : Dry Medium and Prepared Medium 2 - 8°C. | |
Composition** :
| Ingredients | MV1180 Grams/Litre | MV1166 Grams/Litre |
|---|---|---|
| HiVeg hydrolysate | 10.00 | 10.00 |
| Yeast extract | 5.00 | 5.00 |
| Dextrose | 20.00 | 20.00 |
| Sodium acetate | 25.00 | 25.00 |
| Monopotassium hydrogen phosphate | 6.00 | 6.00 |
| Ammonium citrate | 2.00 | 2.00 |
| Polysorbate 80 | 1.00 | 1.00 |
| Magnesium sulphate | 0.575 | 0.575 |
| Manganese sulphate | 0.12 | 0.12 |
| Ferrous sulphate | 0.034 | 0.034 |
| Agar | 15.00 | — |
| Final pH (at 25°C) | 5.5 ± 0.2 | 5.4 ± 0.2 |
** Formula adjusted, standardized to suit performance parameters
Directions :
Suspend 84.7 grams of MV1180 or 69.7 grams of MV1166 in 1000 ml distilled water containing 1.32 ml glacial acetic acid. Heat with frequent stirring. Boil for 1 - 2 minutes to dissolve the medium completely. DO NOT AUTOCLAVE. If storage is necessary, autoclave at 12 lbs pressure (118°C) for 15 minutes.
Principle and Interpretation :
These media are prepared by using HiVeg hydrolysate in place of Casein enzymic hydrolysate which make the medium free of BSE/TSE risks. Lactobacillus Selection HiVeg Agar Base is used for isolation and enumeration of Lactobacilli. Lactobacillus Selection HiVeg Broth Base may be used for isolation and cultivation of Lactobacilli. These media are the modifications of the media developed by Rogosa et al (1, 2) as a selective media for isolation and enumeration of Lactobacilli from oral, faecal specimens (3), food (4) and dairy products (5).
HiVeg hydrolysate, yeast extract and dextrose are the nitrogen and carbon sources. Polysorbate 80 provides fatty acids required for the metabolism of Lactobacilli. Ammonium citrate and sodium acetate inhibit many organisms, including Streptococci, moulds and also restrict swarming. Addition of acetic acid lowers the pH which is inhibitory to many microorganisms but favours the growth of Lactobacilli on the agar medium. Lactobacilli appear as large, white colonies.
Quality Control :
Appearance of Powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel of MV1180.
Colour and Clarity
Yellow coloured, slightly opalescent gel forms in petri plates, clear to slightly opalescent solution in tubes.
Reaction
Reaction of 8.47% w/v aqueous solution of MV1180 is pH 5.5 ± 0.2 at 25°C.
Cultural Response
Reaction of 6.97% w/v aqueous solution of MV1166 is pH 5.4 ± 0.2 at 25°C.
Cultural characteristics observed after an incubation at 35-37°C for 48 hours, in presence of 3 - 5% CO2.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Lactobacillus acidophilus (4356) | 102–103 | luxuriant | >70% |
| Lactobacillus plantarum (8014) | 102–103 | luxuriant | >70% |
| Lactobacillus casei (9595) | 102–103 | luxuriant | >70% |
| Enterococcus faecalis (29212) | 102–103 | inhibited | 0% |
| Proteus vulgaris (13315) | 102–103 | inhibited | 0% |
| Product Name | Lactobacillus Selection HiVeg™ Broth Base |
|---|---|
| SKU | MV1166 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Balows A., Truper H. G., Dworkin M., Harder W., Schleifer K. H., (Eds.), The Prokaryotes, 2nd Edi, 1992, Springer-Verlag2.Wiseman R. F, Sarles W. B, Benton D. A, Harper A. E and Elvehjem C.A., 1956, J. Bacteriol., 72:723.3.Ellis R. F. and Sarles W. B., 1958, J. Bacteriol., 75:272.4.Rogosa M. and Sharpe M. E., 1960, J. Gen. Microbiol., 23:1975.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,American Public Health Association, Washington, D.C.6.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C.7.Rogosa M., Mitchell J. A and Wiseman R. F., 1951, J. Bacteriol.,62:132.8.Rogosa M., Mitchell J. A and Wiseman R. F., 1951, J. Dental Res., 30:682.9.Sabine D. B. and Vaselekos J., 1965, Nature, 206:960.10.MacFaddin J.F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol.1, Williamsand Wilkins, Baltimore. |
| Customized Product Available | No |
Write Your Own Review
Lactobacillus Selection Broth Base
As low as
$256.30
Upsell Products
HiTouch™ Flexi Plate™- TJA
As low as
$183.70