Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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H.S. Vaccine HiVeg™ Medium (Standard Nutrient HiVeg™ Broth)
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$145.20
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Availability: In stock
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SKU:
MV116
A highly nutritive medium recommended for large scale cultivation of bacteriA for production of vaccines.
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Intended Use:
Recommended for large cultivation of bacteria for production of vaccine.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ peptone | 10.000 |
| HiVeg™ extract# | 10.000 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25.00 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
HiVeg™ peptone is the principal source of organic nitrogen while HiVeg™ extract, solid provides carbohydrates, vitamins, organic nitrogen compounds and salts. Sodium chloride maintains osmolality of the medium.
H.S. Vaccine HiVeg™ Medium (Standard Nutrient HiVeg™ Broth) is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks.
Type of specimen
Isolated Microorganism.
Specimen Collection and Handling:
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to light yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Amber coloured clear solution without any precipitate.
Reaction
Reaction of 2.5% w/v aqueous solution at 25°C. pH : 7.6±0.2
pH
7.40-7.80
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | good-luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good-luxuriant |
| Salmonella Typhi ATCC 6539 | 50-100 | good-luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant |
| Staphylococcus epidermidis ATCC 12228 (00036*) | 50-100 | good-luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
Key: (#) Enterobacter aerogenes ATCC 13048 (*) Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | H.S. Vaccine HiVeg™ Medium (Standard Nutrient HiVeg™ Broth) |
|---|---|
| SKU | MV116 |
| Customized Product Available | No |
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