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M-Slanetz Enterococcus HiVeg™ Broth Base

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MV1113

For isolation and detection of Enterococci using membrane filtration technique.

Description

Intended Use

M-Slanetz Enterococcus HiVeg Broth Base is used for isolation and detection of Enterococci using membrane filter technique.

Technical Data

Vegetable based (Code MV)	Animal based (Code M)
MV1113	M1113
HiVeg peptone	Peptic digest of animal tissue
HiVeg hydrolysate	Casein enzymic hydrolysate

Recommended for: Isolation and detection of Enterococci using membrane filter technique.

Reconstitution: 156.0 g/l

Quantity on preparation (500g): 3.20 L

pH (25°C): 7.1 ± 0.2

Supplement: TTC Solution (FD057)

Sterilization: 121°C / 15 minutes.

Storage: Dry Medium - Below 30°C, Use freshly prepared medium.

Composition

Ingredients	Grams/Litre
HiVeg hydrolysate	25.0
HiVeg peptone	15.0
Yeast extract	10.0
Dextrose	2.0
Sucrose	100.0
Dipotassium phosphate	4.0
Sodium azide	0.4
Final pH (at 25°C)	7.1 ± 0.2

Formula adjusted, standardized to suit performance parameters.

Directions

Suspend 156 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool and aseptically add 1 vial of 2,3,5-Triphenyl Tetrazolium Chloride (TTC, FD057). Add 2 ml of the medium on sterile absorbent pad placed in a sterile petri plate.

Warning: Sodium azide has a tendency to form explosive metal azides with plumbing materials. It is advisable to use enough water to flush off the disposables.

Principle and Interpretation

This medium is prepared by using vegetable peptones which makes the medium free of BSE/TSE risks associated with animal based peptones. M-Slanetz Enterococcus HiVeg Broth Base is the modification of M-Slanetz Enterococcus Broth Base which is formulated according to Slanetz and Bartley (1) for isolation and detection of Enterococci using membrane filter technique.

HiVeg hydrolysate, HiVeg peptone and yeast extract provide necessary nutrients like nitrogenous compounds and vitamin B complex. Dextrose and sucrose are the fermentable carbohydrate sources in the medium. Dipotassium phosphate helps in buffering the medium. Sodium azide inhibits the growth of most of the accompanying gram-negative microbial flora. Triphenyl Tetrazolium Chloride is reduced by Enterococci to formazan, a red coloured complex inside the bacterial cell resulting in red coloured colonies.

Quality Control

Appearance of powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.

Colour and Clarity: Yellow coloured, clear solution without any precipitate.

Reaction: Reaction of 15.6% w/v aqueous solution is pH 7.1 ± 0.2 at 25°C.

Cultural Response

Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours.

Organisms (ATCC)	Inoculum (CFU)	Growth	Colour of colony*
Enterococcus faecalis (29212)	10-100	luxuriant	red to maroon
Escherichia coli (25922)	10³	inhibited	-

Key: * = on membrane filter

References

Slanetz L.W. and Bartley C.H., 1957, J. Bact., 74: 591.

Product Information

More Information
Product Name	M-Slanetz Enterococcus HiVeg™ Broth Base
SKU	MV1113
Product Type	HiVeg™
Physical Form	Powder
Origin	Animal Free (Veg)
Packaging type	HDPE
References	1. Eaton A. D., Clesceri L. S. and Greenberg A. E., (Ed.), 1998, Standard Methods for the Examination of Water andWastewater, 20th Ed., American Public Health Association, Washington, D.C.2.Slanetz L. W. and Bartley C. H., 1957, J. Bacteriol., 74: 591.3.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore.4.Slanetz L. W., Bent D. and Bartley C. H., 1955, Public Health Rep.,70: 67.
Customized Product Available	No