Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Salmonella Differential HiVeg™ Agar (Twin pack) (RajHans HiVeg™Medium)
As low as
$42.90
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Availability: In stock
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SKU:
MV1078
For identification and differentiation of Salmonella species from members of Enterobacteriaceae, especially Proteus species.
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Intended Use
Recommended for identification and differentiation of Salmonella species from members of Enterobacteriaceae, especially Proteus species.
Composition**
| Ingredients | g/L |
|---|---|
| Part A | |
| HiVeg® special peptone | 8.000 |
| Yeast extract | 2.000 |
| Synthetic detergent No. III | 1.000 |
| B. C. Indicator | 2.000 |
| Agar | 12.000 |
| Part B | |
| Propylene glycol | 10.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 10 grams of fluid Part B in 1000 ml purified / distilled water. Add 25 grams of Part A. Mix well and heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Salmonella Differential Agar is slight modification of original formulation of Rambach (1) used for differentiation of Salmonella species from Proteus species and other enteric bacteria. Production of acid from propylene glycol is a novel characteristic of Salmonella species and is utilized in these media. Many of the media such as SS Agar, XLD Agar recommended for the identification and differentiation of Salmonella species (2) are based on lactose fermentation and hydrogen sulphide production. Salmonella Differential HiVeg® Agar is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones.
HiVeg® special peptone and yeast extract provides carbonaceous, nitrogenous compounds, long chain amino acids, vitamins and other growth factors supports the luxuriant growth of bacteria. Synthetic detergent No. III inhibits gram-positive organisms rendering the medium selective for enteric microorganisms. The BC indicator turns pink in presence of acid produced from propylene glycol. Lactose fermenting ability is determined by using an indicator, which can detect the presence of enzyme ß-galactosidase. Lactose fermenting (B-galactosidase producing) bacteria yield blue violet coloured colony (3). Salmonellae produce acid from propylene glycol and on combining with the pH indicator gives typical pink red colonies. Other enteric gram-negative bacteria form colourless colonies.
Salmonella Typhimurium and Salmonella Enteritidis produce pink to red colonies. Specimen should be enriched in an appropriate selective enrichment broth. This enriched culture is then inoculated on Salmonella Differential Agar and incubated at 35-37°C for 24-48 hours.
Type of specimen
Water samples and Food samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (4).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (2).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- The medium is selective for Salmonella and may not support the growth of other microorganisms.
- Most of the Salmonella strains shows pink-red colonies except few which may show colourless colonies.
- Due to nutritional variations, some strains may show poor growth.
- Final confirmation of suspected colonies must be carried out by serological and biochemical tests.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Part A: Light yellow to light pink homogeneous free flowing powder Part B: Colourless viscous solution
Gelling
Firm, comparable with 1.2% Agar gel.
Colour and Clarity of Prepared medium
Light orange coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 2.5% w/v aqueous solution of Part A at 25°C. pH: 7.3±0.2
pH
7.10-7.50
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Proteus mirabilis ATCC 25933 | 50-100 | luxuriant | >=50% | colourless |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | >=50% | pink-red |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | luxuriant | >=50% | pink-red |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=50% | blue-green |
| Klebsiella pneumoniae ATCC 13883 (00097*) | 50-100 | luxuriant | >=50% | blue-violet |
| Salmonella Typhi ATCC 6539 | 50-100 | luxuriant | >=50% | colourless |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | luxuriant | >=50% | colourless |
| Staphylococcus aureus Subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% |
Key : (*) Corresponding WDCM numbers
Storage and Shelf Life
Store between 15-25°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Salmonella Differential HiVeg™ Agar (Twin pack) (RajHans HiVeg™Medium) |
|---|---|
| SKU | MV1078 |
| Customized Product Available | No |
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