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Sabouraud Chloramphenicol HiVeg™ Agar
As low as
$291.50
Availability: Out of stock
Availability: In stock
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SKU:
MV1067
for selective cultivation of yeasts and moulds.
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Intended use
Recommended for selective cultivation of yeasts and moulds.
Composition
| Ingredients | g / L |
|---|---|
| HiVeg® hydrolysate | 5.000 |
| HiVeg® peptone | 5.000 |
| Dextrose (Glucose) | 40.000 |
| Chloramphenicol | 0.050 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.6±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 65.05 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Caution: Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet.
Principle And Interpretation
Sabouraud Chloramphenicol Agar is cited as Medium C and recommended for cultivation of yeasts and moulds. This medium was described originally by Sabouraud for the cultivation of fungi, particularly useful for the fungi associated with skin infections. The medium is often used with antibiotics such as Chloramphenicol for the isolation of pathogenic fungi from materials containing large numbers of fungi or bacteria. Sabouraud Chloramphenicol HiVeg® Agar is same as Sabouraud Chloramphenicol Agar except that the animal based peptones are completely replaced with vegetable peptones to avoid the BSE/TSE risks associated with animal peptones. HiVeg® hydrolysate and HiVeg® peptone provide nitrogenous and carbonaceous compounds, long chain amino acids, and other essential growth nutrients. Dextrose provides an energy source. Chloramphenicol inhibits a wide range of Gram-positive and Gram-negative bacteria which makes the medium selective for fungi. The low pH favors fungal growth and inhibits contaminating bacteria from clinical specimens.
Type of specimen
Food and dairy samples.
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines. After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Certain pathogenic fungi may show poor growth on this medium.
- Presence of chloramphenicol may inhibit certain pathogenic fungi.
- Overheating of the medium may result in low productivity and softening of gel.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 6.5% w/v aqueous solution at 25°C. pH : 5.6±0.2
pH: 5.40-5.80
Cultural Response: Cultural characteristics observed after an incubation at 20-25°C for 48-72 hours (Incubate for 7 days for Trichophyton species).
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| # Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | good-luxuriant | |
| Candida albicans ATCC 10231 (00054*) | 50-100 | good-luxuriant | >=50% |
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | 0% |
| **Lactobacillus paracasei ATCC 334 | >=104 | inhibited | 0% |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | good-luxuriant | >=50% |
| Trichophyton rubrum ATCC 28191 | 50-100 | good-luxuriant | |
| Escherichia coli ATCC 8739 (00012*) | >=104 | inhibited | 0% |
Corresponding WDCM numbers, Formerly known as Aspergillus niger, Formerly known as Lactobacillus casei
Storage and Shelf Life
Store the dehydrated powder and prepared medium on receipt between 20-30°C in a tightly closed container. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques.
| Product Name | Sabouraud Chloramphenicol HiVeg™ Agar |
|---|---|
| SKU | MV1067 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Sabouraud K., 1892, Ann. Dermatol. Syphilol, 3:1061. |
| Customized Product Available | No |
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