Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Field's Tryptic digest Broth, HiVeg™ (Tryptic Digest Broth, HiVeg™)
As low as
$254.10
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1028
for cultivation of fastidious microorganisms.
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Intended Use:
Recommended for cultivation of fastidious microorganisms from various samples.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ infusion | 10.000 |
| Sodium chloride | 5.000 |
| Dextrose (Glucose) | 1.000 |
| Final pH (at 25°C) | 7.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 16.0 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile enrichment such as blood, serum or ascitic fluid as required. Mix well and dispense into sterile test tubes or flasks as required.
Principle And Interpretation
Infusion based media are routinely employed for the cultivation of fastidious organisms. These media suffice complex nutritional requirements of most of the organisms. Field (1) formulated Tryptic Digest Broth for cultivation of fastidious microorganisms. Supplementation of the medium with serum, blood or ascitic fluid, it supports the growth of fastidious organisms such as, Neisseria meningitides, Haemophilus influenzae, Streptococcus pneumoniae etc. (2). Shepard et al (3) described its use for culturing Streptococcus pneumoniae and Actinomycetes. Field's Tryptic digest Broth, HiVeg™ is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ infusion provides carbonaceous and nitrogenous compounds and other essential growth nutrients for growth of fastidious microorganisms. Blood serum or ascetic fluid provides additional growth factors.
Type of specimen
Please add specimens
Specimen Collection and Handling:
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to light yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Basal Medium: Light yellow coloured clear solution. After addition of blood, cherry red coloured and with added serum or ascitic fluid, dark yellow coloured, opaque or clear to slightly opalescent solution respectively.
Reaction
Reaction of 1.6% w/v aqueous solution at 25°C. pH: 7.6±0.2
pH
7.40-7.80
Cultural Response
Cultural characteristics observed with added blood, serum or ascetic fluid after incubation at 35-37°C for 24 - 48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Bordetella pertussis ATCC 8467 | 50-100 | luxuriant |
| Haemophilus influenzae ATCC 35056 | 50-100 | luxuriant |
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Field's Tryptic digest Broth, HiVeg™ (Tryptic Digest Broth, HiVeg™) |
|---|---|
| SKU | MV1028 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Field V. J., 1956, SAB Newsletter, 22:8. |
| Customized Product Available | No |
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Field’s Tryptic Digest Broth (Tryptic Digest Broth)
As low as
$254.10