Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
BPL HiVeg™ Agar
As low as
$265.10
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV1020
For isolation and identification of Salmonellae except Salmonella typhi in faeces, urine, milk and other materials.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Recommended for selective isolation and identification of Salmonellae with the exception of Salmonella serotype Typhi in meat, milk and other materials.
Composition**
| Ingredients | g / L |
|---|---|
| HiVeg™ peptone No. 1 | 7.000 |
| Sodium chloride | 5.000 |
| Lactose | 15.000 |
| Phenol red | 0.040 |
| Brilliant green | 0.005 |
| Agar | 13.000 |
| Final pH (at 25°C) | 6.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40.04 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Brilliant green, phenol red, lactose Agar (BPL) is a selective agar medium for the identification and isolation of Salmonella with the exception of Salmonella Typhi in faeces, urine, meat, milk and other materials (1). BPL HiVeg™ Agar is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. The medium contains HiVeg™ peptone No. 1, which supplies the nitrogenous nutrients to the organisms. Lactose is the fermentable carbohydrate, which after degradation yields acid production, indicated by the phenol red indicator. In the acidic range, phenol red turns yellow while in alkaline conditions it turns red. Brilliant green inhibits gram-positive organisms and also Salmonella Typhi and Shigella species.
Type of specimen
Food and dairy samples; Water samples
Specimen Collection and Handling:
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (2,3,4). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(5) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical tests must be performed for confirmation.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm, comparable with 1.3% Agar gel.
Colour and Clarity of prepared medium
Brownish green coloured, clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 4.0% w/v aqueous solution at 25°C. pH: 6.5±0.2
pH
6.30-6.70
Cultural Response
Cultural characteristics observed in a humid atmosphere after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| **Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | none-poor | <=10% | |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | none-poor | <=10% | |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | poor-good | 30-40% | yellow |
| Salmonella Choleraesuis ATCC 12011 | 50-100 | good-luxuriant | >=50% | pink-red |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | good-luxuriant | >=50% | pink-red |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | good-luxuriant | >=50% | pink to red |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% |
Key: *Corresponding WDCM numbers **- Formerly known as Bacillus subtilis subsp. spizizenii.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | BPL HiVeg™ Agar |
|---|---|
| SKU | MV1020 |
| Customized Product Available | No |
Write Your Own Review