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Dichloran HiVeg™ Medium Base with Rose Bengal

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SKU:
MV1000
For selective isolation and enumeration of yeasts and moulds associated with food spoilage.

Intended Use

Dichloran HiVeg™ Medium Base with Rose Bengal is used for selective isolation and enumeration of Fungi-yeasts and moulds of significance in food spoilage.

Composition

Ingredients Grams/Litre
HiVeg peptone 5.0
Dextrose 10.0
Monopotassium phosphate 1.0
Magnesium sulphate 0.5
Rose Bengal 0.025
Dichloran 0.002
Agar 15.0

Final pH (at 25°C) 5.6 ± 0.2

** Formula adjusted, standardized to suit performance parameters.

Directions

Suspend 15.76 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add sterile reconstituted contents of 1 vial of Chloramphenicol Selective Supplement (FD033). Mix well and pour into sterile petri plates.

Principle and Interpretation

Dichloran HiVeg Medium Base with Rose Bengal is prepared by replacing animal based peptone with vegetable peptones which is free from BSE/TSE. Dichloran HiVeg Medium Base with Rose Bengal is the modification of Dichloran Medium Base with Rose Bengal which is formulated as described by King et al (1), which is a modification of Rose Bengal Chloramphenicol Agar (2). It is recommended for the selective isolation and enumeration of yeasts and moulds of importance in food spoilage. Dichloran HiVeg Medium Base with Rose Bengal is the modification of this medium by replacing animal based peptone with vegetable peptones and it serves the same purpose.

Dichloran inhibits spreading of moulds such as Rhizopus and Mucor. Chloramphenicol inhibits bacterial growth. Dichloran HiVeg Medium Base with Rose Bengal is used along with Rose Bengal Chloramphenicol Agar where it is necessary to enumerate yeasts in the presence of moulds. Add 40 ml of food sample to 200 ml of 0.1% HiVeg Peptone water (MV028) and shake periodically for 30 minutes (3) or process in stomacher for 30 seconds (4). Inoculate 0.1 ml of this sample on Dichloran HiVeg Medium Base with Rose Bengal. Report the number of colonies per gram of food.

Product Profile

Vegetable based (Code MV) Animal based (Code M)
MV1000 M1000
HiVeg peptone Peptic digest of animal tissue

Recommended for : Selective isolation and enumeration of fungi, yeasts & moulds.

Reconstitution : 31.5 g/l

Quantity on preparation (500g) : 15.87 L

pH (25°C) : 5.6 ± 0.2

Supplement : Chloramphenicol Selective Supplement (FD033)

Sterilization : 121°C / 15 minutes.

Storage : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.

Quality Control

Appearance of powder
Light pink coloured, homogeneous, free flowing powder.

Gelling
Firm, comparable with 1.5% Agar gel.

Colour and Clarity
Pink coloured, clear to slightly opalescent gel forms in petri plates.

Reaction
Reaction of 3.15% w/v aqueous solution is pH 5.6 ± 0.2 at 25°C.

Cultural Response

Cultural characteristics observed after an incubation at 25-30°C for 5 days, on addition of 1 vial of Chloramphenicol Selective Supplement (FD033).

Organisms (ATCC) Growth
Bacillus subtilis (6633) inhibited
Candida albicans (10231) good
Escherichia coli (25922) inhibited
Mucor racemosus (42647) good
Saccharomyces cerevisiae (9763) good-luxuriant

References

  1. King D.A. Jr., Hocking A.D. and Pitt J.I., 1979, J. Appl. Environ. Microbiol., 37:959.
  2. Jarvis B., 1973, J. Appl. Bact., 36:723.
  3. Sharf J.M. (Ed.), 1966, American Public Health Association, 2nd ed., New York.
  4. Sharp A.N. and Jackson A.K., 1972, J. Appl. Bact., 24:175.
More Information
Product Name Dichloran HiVeg™ Medium Base with Rose Bengal
SKU MV1000
Product Type HiVeg™
Physical Form Powder
Origin Animal Free (Veg)
Packaging type HDPE
References 1. King D.A. Jr., Hocking A.D. and Pitt J.I., 1979, J. Appl. Environ. Microbiol., 37:959.2.Jarvis B., 1973, J. Appl. Bact., 36:723.3.Sharf J.M. (Ed.), 1966, American Public Health Association, 2nd ed., New York.4.Sharp A.N. and Jackson A.K., 1972, J. Appl. Bact., 24:175.
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