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Nutrient HiVeg™ Agar, pH 6.0 w/ 0.8% NaCl

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MV090
For cultivation of bacteria requiring slightly acidic pH.

Intended Use

Nutrient HiVeg Agar Medias are recommended as general purpose culture media for microbiological analysis of water and cultivation of bacteria requiring slightly acidic pH.

Composition

Ingredients MV1269 Grams/Litre MV090 Grams/Litre
HiVeg peptone 10.00 5.00
HiVeg extract 10.00 3.00
Sodium chloride 5.00 8.00
Agar 15.00 15.00
Final pH (at 25°C) 7.2 ± 0.2 6.0 ± 0.2

Formula adjusted, standardized to suit performance parameters.

Product Profile

Vegetable based (Code MV)

  • MV1269/MV090
  • HiVeg peptone
  • HiVeg extract

Animal based (Code M)

  • M1269/M090
  • Peptic digest of animal issue
  • Beef extract

Technical Specifications

Reconstitution:

  • (MV1269): 40.0 g/l
  • (MV090): 31.0 g/l

Quantity on preparation (500g):

  • (MV1269): 12.5 L
  • (MV090): 16.12 L

Quantity on preparation (100g):

  • (MV090): 3.22 L

Supplement: None

Sterilization: 121°C / 15 minutes.

Storage: Dry Medium Below 30°C, Prepared Medium 2 - 8°C.

Directions

Suspend 40 grams of MV1269 or 31 grams of MV090 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Principle and Interpretation

These media are prepared by completely replacing animal based peptones with vegetable peptones, which makes the media free of BSE/TSE risks. HiVeg peptone and HiVeg extract are used in these medias as veg substitute in place of Peptic digest of animal tissue and Beef extract respectively.

Nutrient HiVeg Medias are the modification of Nutrient Media which are general purpose media used for the examination of water and dairy products according to Standard Methods for the Examination of Water and Wastewater (1) and Dairy Products (2). Nutrient HiVeg Agar No. 2 like the conventional Nutrient Agar No.2 can be used for the Microbiological analysis of water as per Czech Standards. It can also be used for sterility testing of aerobes and maintenance of subcultures (3). Nutrient HiVeg Agar with 0.8% NaCl and pH 6.0 which is a modification of Nutrient Agar w/ 0.8% NaCl and pH 6.0 recommended by APHA for the growth of microorganisms requiring slightly acidic conditions.

HiVeg peptone and HiVeg extract provides the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains osmotic equilibrium of the medium.

Quality Control

Appearance of powder: Light yellow to yellow coloured may have slightly greenish tinge, homogeneous, free flowing powder.

Gelling: Firm, comparable with 1.5% Agar gel of MV1269 or MV090.

Colour and Clarity: Light yellow to amber coloured, clear to slightly opalescent gel forms in petri plates.

Reaction

Reaction of 4.0% w/v aqueous solution of MV1269 is pH 7.2 ± 0.2 at 25°C. Reaction of 3.1% w/v aqueous solution of MV090 is pH 6.0 ± 0.2 at 25°C.

Cultural Response

Cultural characteristics observed after an incubation at 35 - 37°C for 18-24 hours of MV1269.

Organisms (ATCC) Inoculum (CFU) Growth Recovery
Escherichia coli (25922) 102-103 luxuriant >70%
Enterobacter aerogenes (13048) 102-103 luxuriant >70%
Salmonela serotype Typhimurium (14028) 102-103 luxuriant >70%
Klebsiella pneumoniae (13883) 102-103 luxuriant >70%

Cultural characteristics observed after an incubation at 35 - 37°C for 18-24 hours of MV090.

Organisms (ATCC) Inoculum (CFU) Growth Recovery
Bacillus subtilis (6633) 102-103 good >50%
Candida albicans (10231) 102-103 luxuriant >70%
Staphylococcus aureus (25923) 102-103 good >50%

References

  1. Greenberg A.E., Trussell R.R. and Clesceri L.S. (Eds.), 1985, Standard Methods for the Examination of Water and Wastewater, 16th ed., APHA, Washington, D.C.
  2. American Public Health Association, 1978, Standard Methods for the Examination of Dairy Products, 14th ed., APHA, Inc., Washington, D.C.
  3. MacFaddin, J. (1985); Methods for Isolation-Cultivation- Identification-Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.
  4. Downes FP and Ito K (Eds.), 2001, Compendium of Methods For The Microbiological Examination of Foods, 4th ed., APHA, Washington, D.C.
More Information
Product Name Nutrient HiVeg™ Agar, pH 6.0 w/ 0.8% NaCl
SKU MV090
Product Type HiVeg™
Physical Form Powder
Origin Animal Free (Veg)
Packaging type HDPE
References 1. Clesceri L. S, Greenberg A. E. and Eaton A. D., (Eds.), 1998, Standard Methods for the Examination of Water andWastewater, 20th Ed., APHA, Washington, D.C.
2.American Public Health Association, 1978, Standard Methods for the Examination of Dairy Products, 14th Ed., APHA,Inc., Washington, D.C.
3.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,American Public Health Association, Washington, D.C.
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