Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
MacConkey HiVeg™ Agar w/ Bromo Thymol Blue
As low as
$48.40
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV061
For detection of lactose fermenting enteric bacteria.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Recommended for detection of lactose fermenting enteric bacteria from various samples.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg® peptone | 17.000 |
| HiVeg® peptone No. 3 | 3.000 |
| Lactose | 10.000 |
| Synthetic detergent | 1.500 |
| Sodium chloride | 5.000 |
| Bromothymol blue | 0.030 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 51.53 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
MacConkey Agar is the earliest selective and differential medium for cultivation of enteric microorganisms from a variety of clinical specimens (1,2). The original medium contains protein, bile salts, sodium chloride and two dyes. MacConkey Agar w/ Bromo thymol blue is a modification of the original medium by the replacement of the two dyes with a single dye i.e. bromo thymol blue. MacConkey HiVeg® Agar w/ Bromo Thymol Blue is same as MacConkey Agar w/ Bromo Thymol Blue except that the animal based peptones are completely replaced with vegetable peptones to avoid the BSE/TSE risks associated with animal peptones.
HiVeg® peptone and HiVeg® peptone No. 3 serve as the sources of essential nutrients. Lactose is the fermentable carbohydrate source. Lactose fermenting enteric bacteria ferment lactose and produce acidic byproducts. This acidic condition formed causes the pH indicator dye i.e. bromo thymol blue to change colour from blue to yellow. Lactose non-fermenters fail to cause a colour change in the medium. Sodium chloride maintains the osmotic equilibrium of the medium. Synthetic detergent serves to make the medium selective by inhibiting the accompanying gram-positive bacteria.
Type of specimen
Food and dairy samples; Water samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3,4,5). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to greenish yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Green coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 5.15% w/v aqueous solution at 25°C. pH: 7.1±0.2
pH
6.90-7.30
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) |
Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | luxuriant | >=50% | yellow |
| Enterococcus faecalis ATCC 29212 (00087*) | >=104 | inhibited | 0% | |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=50% | yellow |
| ## Proteus hauseri ATCC 13315 | 50-100 | luxuriant | >=50% | colourless-light blue |
| Salmonella Typhi ATCC 6539 | 50-100 | luxuriant | >=50% | colourless-light blue |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | luxuriant | >=50% | colourless-light blue |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% |
Key: (*) Corresponding WDCM numbers.
(#) Formerly known as Enterobacter aerogenes
(##) Formerly known as Proteus vulgaris
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | MacConkey HiVeg™ Agar w/ Bromo Thymol Blue |
|---|---|
| SKU | MV061 |
| Customized Product Available | No |
Write Your Own Review