Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Alternative Thioglycollate HiVeg® Medium, Sterile Powder
As low as
$179.30
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV010G
Recommended for evaluation of sterility in manufacturing process.
Your enquiry has been submitted
Please login to Post an Enquiry.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiVeg® hydrolysate | 15.000 |
| Yeast extract | 5.000 |
| Dextrose(Glucose) | 5.500 |
| Sodium chloride | 2.500 |
| L-Cystine | 0.500 |
| Sodium thioglycollate | 0.500 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Sterile powder can be used directly for the evaluation of sterility in manufacturing process. For sterile liquid medium aseptically add 29.0 grams in 1000 ml sterile distilled / purified water. Heat if necessary to dissolve the medium completely.Dispense aseptically in sterile tubes or flasks as desired. DO NOT AUTOCLAVE OR OVERHEAT. Excessive heating is detrimental. (Sterilised by gamma irradiation)
Note:
It is preferable to use freshly prepared medium, alternatively it should be boiled and cooled just once prior to use or with reheating, toxic oxygen radicles are formed.
Principle And Interpretation
Alternative Thioglycollate HiVeg® Medium, sterile powder is prepared by replacing animal based Pancreatic digest of casein of Alternative Thioglycollate Medium, sterile powder with plant based HiVeg® Hydrolysate. This makes the medium free of BSE-TSE associated risks. The formula is based on N.I.H. Memorandum (1), U.S. Pharmacopoeia (2) and Indian Pharmacopoeia (3). Alternative Thioglycollate HiVeg® Medium contains sodium thioglycollate that can neutralize the bacteriostatic effect of mercurial preservatives. Absence of agar makes it suitable for testing viscous materials and devices having tubes with small lumina. HiVeg® hydrolysate, yeast extract, dextrose, L-Cystine provides nitrogenous and carbonaceous compounds, vitamin B complex, trace elements and other essential growth nutrients.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured clear solution without any precipitate.
Reaction: Reaction of 2.9% w/v aqueous solution at 25°C. pH: 7.1±0.2
pH: 6.90-7.30
Sterility Testing: No growth is observed after 14 days for Bacteria at 30-35°C and for Fungi at 20-25°C. No growth of Mycoplasma after 14 days at 35-38°C under microaerophilic condition
Stability test: Light yellow coloured clear solution without any precipitation or sedimentation at 30-35° for 7 days
Cultural response
Cultural characteristics observed after an incubation at 30-35°C for not more than 3 days.
Cultural Response
| Organism | Growth | Inoculum (CFU) |
|---|---|---|
| *Clostridium sporogenes ATCC 19404 | luxuriant | 50-100 |
| *Clostridium sporogenes ATCC 11437 | luxuriant | 50-100 |
| *Clostridium sporogenes NBRC 14293 | luxuriant | 50-100 |
| *Clostridium perfringens ATCC 13124 | luxuriant | 50-100 |
| Staphylococcus aureus ATCC25923 | luxuriant | 50-100 |
| *Bacteroides fragilis ATCC 23745 | luxuriant | 50-100 |
| *Bacteroides vulgatus ATCC 8482 | luxuriant | 50-100 |
| Pseudomonas aeruginosa ATCC 27853 | luxuriant | 50-100 |
| Pseudomonas aeruginosa ATCC 9027 | luxuriant | 50-100 |
| Escherichia coli ATCC 25922 | luxuriant | 50-100 |
| Escherichia coli ATCC 8739 | luxuriant | 50-100 |
| Salmonella Abony NCTC 6017 | luxuriant | 50-100 |
| Salmonella Typhimurium ATCC 14028 | luxuriant | 50-100 |
| Staphylococcus aureus ATCC 6538 | luxuriant | 50-100 |
| Escherichia coli NCTC 9002 | luxuriant | 50-100 |
| #Candida albicans ATCC 10231 | luxuriant | 50-100 |
Key: (*) Incubated anaerobically (#) Incubated at 20-25°C
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Alternative Thioglycollate HiVeg® Medium, Sterile Powder |
|---|---|
| SKU | MV010G |
| Product Type | HiVeg™ |
| Physical Form | Sterile Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. N.I.H. Memorandum, 1955: Culture Media for Sterility Tests, 4th |
| Customized Product Available | No |
Write Your Own Review
Alternative Thioglycollate Medium, Sterile Powder
As low as
$179.30
Alternative Thioglycollate Medium, Sterile Powder
As low as
$0.00
Upsell Products
Alternative Thioglycollate Medium
As low as
$88.00