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Antibiotic HiVeg™ Assay Medium No.1 (Seed HiVeg™ Agar)
As low as
$51.70
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV003
For microbiological assay of ß-lactam and other antibiotics in pharmaceutical and food related preparations. HiVeg medium equivalent to M003.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiVeg peptone | 6.000 |
| HiVeg hydrolysate | 4.000 |
| Yeast extract | 3.000 |
| HiVeg extract | 1.500 |
| Dextrose | 1.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.5 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Advice : Recommended for the microbiological assay of Bacitracin, Cephalexin, Cephaloglycin, Cephradine, Cephaloridine, Cephalothin, Cephaperin, Cephazolin, Cloxacillin Cycloserine, Dicloxacillin, Methicllin, Nafcillin, Novobiocin, Oxacillin, Penicillin-G and Phenoxymethyl Penicillin .
Principle And Interpretation
Antibiotic HiVeg Assay Medium No.1 (Seed HiVeg Agar) is prepared by replacing animal based peptones with vegetable peptones, making the medium BSE- TSE risks free. It can be used for the same purpose of Antibiotic Assay Medium No.1 (Seed Agar). The potency of an antibiotic can be determined by chemical, physical and biological assays. Biological assays offer the most convenient method (1), since a reduction in the antimicrobial activity of a specific antibiotic is not usually displayed in chemical methods (2). Biological testing may be performed by either dilution (turbidimetric) or diffusion methods. The choice of methodology is often based on many factors, including relative ease of performance, flexibility and use of automated or semi-automated devices for both identification and susceptibility testing (3). Grove and Randall have elucidated those antibiotic assays and media in their comprehensive treatise on antibiotic assays (4). Antibiotic HiVeg Assay Medium No.1 is used in the microbiological assay of ß-lactam and other antibiotics and as seed agar with Micrococcus luteus (ATCC9341) for plate assay of Bacitracin, with Staphylococcus aureus (ATCC 29739) for cylinder plate assay of Cephalexin, Cephalothin, Cephapirin, Cloxacillin, Dicloxacillin, Methicillin, Nafcillin, Oxacillin, Penicillin-G and Staphylococcus epidermidis (ATCC 12228) for plate assay of Novobiocin. This media can be used according to the specifications detailed in various pharmacopoeias (2,5,6) and by the FDA (7).
Antibiotic assays are normally performed in freshly prepared media. Test organisms are spread evenly over the surface of solidified base agar. After incubation, the concentration of the antibiotic being assayed is determined by measuring the zone of inhibition obtained, with that of reference standard antibiotic. All conditions in the microbiological assay must be carefully controlled. The use of standard culture media in the test is one of the important steps for good results.
Nutrients and growth factors are supplied by the HiVeg peptone, HiVeg hydrolysate, yeast extract and HiVeg extract. Dextrose is supplemented as a carbon and energy source.
Note: For Antibiotic Assay Methods and Selection of Antibiotic HiVeg Assay Medias, Refer Section Antibiotic HiVeg Assay Media.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of Prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petriplates.
Reaction
Reaction of 3.05% w/v aqueous solution at 25°C. pH: 6.6±0.2
pH
6.40-6.80
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Inoculum medium | Assay medium Inoculum & Assay medium |
|---|---|---|---|---|---|
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | >70% | Framycetin, Josamycin, Josamycin propionate, Kanamycin B, Spiramycin, Streptomycin | Streptomycin |
| Bordetella bronchiseptica ATCC 4617 | 50-100 | luxuriant | >50% | Colistimethate sodium, Colistin, Polymyxin B | |
| Escherichia coli ATCC 10536 | 50-100 | luxuriant | >70% | Chloramphenicol | |
| Bacillus cereus var mycoides ATCC 11778 | 50-100 | luxuriant | >70% | Oxytetracycline, Tetracycline | |
| Bacillus pumilis ATCC 14884 | 50-100 | luxuriant | >70% | Chlortetracycline, Framycetin, Kanamycin sulphate | |
| Klebsiella pneumoniae ATCC 10031 | 50-100 | luxuriant | >70% | Capreomycin, Dihydrostreptomycin, Neomycin, Streptomycin, Troleandomycin | |
| Micrococcus luteus ATCC 9341 | 50-100 | luxuriant | >70% | Erythromycin, Rifamycin | Erythromycin |
| Micrococcus luteus ATCC 10240 | 50-100 | luxuriant | >70% | Bacitracin | |
| Pseudomonas aeruginosa ATCC 25619 | 50-100 | luxuriant | >70% | Carbenicillin | |
| Staphylococcus aureus ATCC 29737 | 50-100 | luxuriant | >70% | Amikacin, Cephalothin, Cephapirin, Cloxacillin, Chlortetracycline, Nafcillin, Penicillin-G, Cycloserine, Doxycycline, Demeclocycline, Methacycline, Oxytetracycline, Rolitetracycline, Tetracycline, Tobramycoin, Tylosin | |
| Staphylococcus epidermidis ATCC 12228 | 50-100 | luxuriant | >70% | Gentamycin, Netilmycin, Neomycin, Novobiocin, Sisomycin, Paromomycin | Novobiocin |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on label.
| Product Name | Antibiotic HiVeg™ Assay Medium No.1 (Seed HiVeg™ Agar) |
|---|---|
| SKU | MV003 |
| Application/ Industry | Pharmaceutical |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Pelczar M. J. Jr., Reid R. D., Chan E. C. S., 1977, Microbiology, 4th Edi, Tata McGraw-Hill Publishing Company Ltd,New Delhi 2. The United States Pharmacopoeia, 2018, The United States Pharmacopoeial Convention,Rockville, MD. 3. Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. 4. Grove and Randall, 1955, Assay Methods of Antibiotics Medical Encyclopedia, Inc.New York.5.European Pharmacopoeia, 2017, European Department, for the Quality of Medicines6.British Pharmacopoeia, 2016,The Stationery office British Pharmacopoeia7.Tests and Methods of Assay of Antibiotics and Antibiotic containing Drugs, FDA, CFR, 1983 Title 21, Part 436, SubpartD, Washington, D.C.: U.S. Government Printing Office, paragraphs 436, 100-436, 106, p. 242- 259 (April 1).8.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.9.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
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