Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Modified Middlebrook 7H9 Broth with Indicator
As low as
$140.80
Availability: Out of stock
Availability: In stock
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SKU:
MT001
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Reagent supplied as single unit ready to use two component system.
- Middle brook 7H9Broth base.
- Modified Selective Enrichment (Lyophilized).
Directions
Preparation of Modified Middlebrook 7H9 broth w/Indicator.
- Reconstitute Modified Selective Enrichment with one ml of Sterile distilled water.
- Transfer reconstituted Modified Selective Enrichment aseptically to the Middlebrook 7H9 broth base. This is now ready to use as Modified Middlebrook 7H9 broth w/indicator Medium.
Test Protocol
- Inoculate aseptically 50 -300 µl of decontaminated & concentrated specimen in the Modified Middlebrook 7H9 Broth w/Indicator, using calibrated device (Inoculum should be inversely proportional to the initial M.T.B.Count in the specimen). Mix well.
- Incubate at 35-37°C observe for the growth every third day.
Principle And Interpretation
Modified Middlebrook 7H9 Broth w/ Indicator is the modification of Middlebrook 7H9 broth developed by Middlebrook etal (1). Growth promoting factor incorporated in the medium, accelerates growth and presence of biological indicatorit possible to visualize growth distinctly as early as 6 days and on an average of 10-14 days in most of the case. Antibiotic cocktail (PACT) present in the system eliminates/ retards growth of contaminating flora. This medium contains many inorganic salts which support the growth of Mycobacteria Sodium citrate becomes citric acid in the medium which retains certain inorganic cations in the solution. This broth base is supplemented for the growth of Mycobacteria. Enrichment Supplement contains bovine albumin, dextrose, catalase and sodium chloride. Some free fatty acids are toxic to Mycobacteria but albumin binds to those fatty acids and prevent toxic action on Mycobacteria. Toxic peroxides present in the medium are destroyed by catalase. Dextrose supplies energy while sodium chloride maintains osmotic equilibrium. Glycerol enhances the growth
Quality Control
Appearance
Reagent is supplied as single unit ready to use two component system.
- Middlebrook 7H9 Broth base-Light yellow coloured clear solution.
- Modified Selective Enrichment Supplement(Lyophilized) - Beige to light pink powder.
Cultural response
Cultural response observed after incubation at 35-37°C till 2-4 weeks(observe for growth every third day)
Mycobacterium tuberculosis H37RV (25618)
Magenta pink coloured granular deposition(To be confirmed with microscopy and other tests)
Sterility test
Passes release criteria
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label.
| Product Name | Modified Middlebrook 7H9 Broth with Indicator |
|---|---|
| SKU | MT001 |
| Customized Product Available | No |
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