Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiCulture™ Transport Swab w/ Soyabean Casein Digest Medium (γ-irradiated) (Triple pack)
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SKU:
MS011GT
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Intended Use
Recommended for the sterility testing in clean area
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Dextrose (Glucose) | 2.500 |
| Dipotassium hydrogen phosphate | 2.500 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Using the capped swab provided along with the medium containing tube, collect the sample or specimen from surface. Discard the cap of the tube and insert the capped swab with the sample till the bottom of the medium. Tighten the cap firmly. The specimen will be preserved during transportation and also the viability of the organisms will be maintained but it will diminish over the time. Some growth of contaminants may occur during longer period of transport. After the transportation, the specimen should be inoculated in proper medium as soon as possible. The cultures on transport swabs must not be kept at room temperature for more than 24 hours.
Principle And Interpretation
Soyabean Casein Digest Medium is recommended by various pharmacopeias as a sterility testing and as a microbial limit testing medium (2,5,6). This medium is a highly nutritious medium used for cultivation of a wide variety of organisms (1). The combination of Tryptone and soya peptone makes the medium nutritious by providing nitrogenous, carbonaceous substances, amino acids and long chain peptides for the growth of microorganisms. Dextrose/glucose serve as the carbohydrate source and dibasic potassium phosphate buffer the medium. Sodium chloride maintains the osmotic balance of the medium.
Type of specimen
Environmental monitoring
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Soyabean Casein Digest Medium in tubes with sterile viscous swabs (y irradiated) (Triple pack)
Colour
Yellow coloured medium
Quantity of Medium
5ml of medium in tubes
Reaction
7.10-7.50
Dose of Irradiation (Kgy)
25.00-30.00
Sterility check
Passes release criteria
Cultural response
Viability of following organisms was established for a period of 48 hours. Organisms grew luxuriantly when recovered on Tryptone Soya Agar (M290) and incubated at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Recovery |
|---|---|---|
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant |
| Salmonella Abony NCTC 6017 (00029*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 9027 (00026*) | 50-100 | luxuriant |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant |
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant |
| # Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant |
Key: (#) Formerly known as Aspergillus niger, (*) Corresponding WDCM numbers
Storage and Shelf Life
Store between 5-25°C. Use before expiry date on the label.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | HiCulture™ Transport Swab w/ Soyabean Casein Digest Medium (γ-irradiated) (Triple pack) |
|---|---|
| SKU | MS011GT |
| Customized Product Available | No |
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