This medium is recommended for selection and subculture of Clostridium sporogenes in accordance with the harmonized method of USP/EP/BP/JP/IP. This medium is highly nutritious as it contains tryptone, HM extract and HM hydrolysate and yeast extract provides carbonaceous and nitrogenous substances, long chain amino acids, vitamins of B complex group and other essential nutrients for the luxuriant growth of fastidious as well as non-fastidious organisms.