Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiCrome™ Strep B Selective Agar Plate
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SKU:
MP1966
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Intended use
Recommended for selective isolation of Group B streptococci from clinical sample.
Composition
| Ingredients | g / L |
|---|---|
| Peptone special | 10.000 |
| Yeast extract | 4.300 |
| Chromogenic mixture | 7.500 |
| Phenol red | 0.025 |
| Agar | 15.000 |
GCN Selective Supplement (FD273)
| Ingredients | Concentration |
|---|---|
| Colistin | 10mg |
| Nalidixic acid | 10mg |
| Gentamicin | 2mg |
Final pH (at 25°C) 7.4±0.2
Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Group B Streptococcus is a leading infection causing illness and death in newborns. Group B streptococci can also cause serious diseases in pregnant women, the elderly, and adults with other illnesses. GBS normally reside in the vagina of women and rectum of men and women (1). In newborns, group B strep is the most common cause of sepsis (infection of the bloodstream) and meningitis (infection of the lining and fluid surrounding the brain) and a common cause of pneumonia. In adults, group B strep can rarely lead to serious bloodstream infections, urinary tract infections, skin infections, and pneumonia, especially in people with weak immune systems. Heavy colonization of the maternal genital tract is associated with colonization of infants and risk of neonatal disease (2).
The sample collection is usually done by collection of vaginal and rectal swab between 35 and 37 weeks of pregnancy. The swab is then processed on HiCrome™ Strep B Selective Agar Base. For the conventional methods optimum recovery is however achieved by selective enrichment into Todd Hewitt broth with colistin and nalidixic acid and then subculture on Blood Agar (3,4).
Peptone special and yeast extract provides nitrogeneous and carbonaceous compounds, long chain amino acids, vitamins and essential nutrients for the growth of Streptococci. One of the chromogenic substrate is utilized by Group B Streptococci resulting in purple coloured colonies, while the other Streptococci either give blue or bluish green coloured colonies with yellow background. Phenol red is the indicator dye.
Type of specimen
Clinical samples: Vaginal and rectal samples, urine, etc.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Slight colour variation may be observed depending upon the utilization of the substrate by the organism.
- Further biochemical tests must be carried out for confirmation.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile HiCrome™ Strep B Selective Agar, Modified in 90mm disposable plate with smooth surface and absence of black particles/cracks/bubbles
Colour: Reddish orange coloured medium
Quantity of medium: 25 ml of medium in 90 disposable mm plate
pH: 7.20-7.60
Sterility Check: Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24 - 48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | 0% | |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% | |
| Streptococcus agalactiae ATCC 13813 | 50-100 | luxuriant | >=50% | purple |
| Enterococcus faecalis ATCC 29212 (00187*) | 50-100 | luxuriant | >=50% | bluish green |
| Enterococcus faecium ATCC 19434 | 50-100 | luxuriant | >=50% | green w/yellow background |
Key- (*) Corresponding WDCM numbers
Storage and Shelf Life
Store between 2-8°C. Use before expiry period on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | HiCrome™ Strep B Selective Agar Plate |
|---|---|
| SKU | MP1966 |
| Customized Product Available | No |
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