2X Tris-Tricine Gel Loading Buffer

Product Identifier

Introduction:

2X Tris-Tricine gel loading buffer is specifically used for protein sample preparation prior to loading on Tris-Tricine- SDS-PAGE. It is added to each protein sample at 1:1 ratio and boiled for 1-5 min. The use of Tris-Tricine gel loading buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-Tricine-SDS gel running buffer.

Description:

2X Tris-Tricine gel loading buffer is used as a protein sample loading buffer for use in Tris-Tricine-SDS-PAGE and Western Blotting. This buffer contains Coomassie Brilliant Blue G-250 (CBB G-250), SDS, glycerol and Tris-HCl. The CBB G-250 serves as an indicator dye visually facilitating loading of samples into the wells of the gel. It also functions as a migration dye helping to monitor the dye front that runs ahead of the proteins. Glycerol in the buffer increases the density of the sample so that it will fall to the bottom of the well, minimizing puffing or loss of protein sample in the buffer, and results in convenient layering of the samples into the wells. The SDS detergent binds to the positive charges occurring on the proteins at a regular interval, thus giving each protein the same overall negative charge. Hence the proteins will separate based on size and not by charge. The SDS also denatures the proteins and subunits to help them separate on basis of their size, irrespective of shape.

Application:

2X Tris-Tricine gel loading buffer is specifically used for protein sample loading during Tris-Tricine-SDS-PAGE for separation of lower molecular weight proteins or peptides.

Note:

Tricine Sample Loading Buffer does not contain reducing agent. For reducing conditions, add 20µl of 2-Mercaptoethanol to 980µl of Tricine gel loading Buffer (2X) before use for a final concentration of 2% or 19.3mg of DL-DTT to 1 mL of Tricine gel loading buffer. For best results, do not store sample with 2-Mercaptoethanol after use.

Composition:

2X Tris-Tricine Gel Loading Buffer is made from molecular biology grade Coomassie Brilliant Blue G-250, SDS, glycerol, Tris-CL and conveniently premixed to save time. It is supplied as ready to use solution.

Storage conditions:

2X Tris-Tricine Gel Loading Buffer has to be stored at room temperature (15-25 °C). Under recommended condition, the reagent is stable for 6 months.

Warning and Precautions

Not for Medicinal Use. Read the SDS carefully before beginning the protocol. Wear protective gloves/protective clothing/eye protection/face protection. Follow good clinical laboratory practices while handling clinical samples. Standard precautions should be followed as per established guidelines. Safety guidelines may be referred in safety data sheets of the product.

Performance and Evaluation

Performance of the solution is expected when the solution is stored at recommended temperature and within the expiry period.

Safety Information

The 2X Tris-Tricine Gel Loading Buffer is for laboratory use only, not for drug, household or other uses. Take appropriate laboratory safety measures and wear gloves and safety goggles while handling. Not compatible with disinfecting agents containing bleach. Please refer the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.

Technical Assistance

At HiMedia we pride ourselves on the quality and availability of our technical support. For any kind of technical assistance, mail at mb@himedialabs.com.