2X Tris-Tricine Gel Loading Buffer

2X Tris‐Tricine gel loading buffer is specifically used for protein sample preparation prior to loading on Tris‐Tricine‐ SDS‐PAGE. It is added to each protein sample at 1:1 ratio and boiled for 1‐5 min. The use of Tris‐Tricine gel loading buffer ensures optimal band resolution when preparing proteins for SDS‐PAGE with Tris‐Tricine‐SDS gel running buffer. 2X Tris-Tricine Gel Loading Buffer prepares small proteins and peptides for high resolution electrophoresis. This loading buffer ensures proper sample density for peptide separation and low molecular weight analysis in molecular protein research testing.