2X Tris-Tricine gel loading buffer is specifically used for protein sample preparation prior to loading on Tris-Tricine- SDS-PAGE. It is added to each protein sample at 1:1 ratio and boiled for 1-5 min and loaded.
2X Tris-Tricine gel loading buffer is used as a protein sample loading buffer for use in Tris-Tricine-SDS-PAGE for separation of lower molecular weight proteins or peptides. . This buffer contains Coomassie Brilliant Blue G-250 (CBB G-250), SDS, glycerol and Tris-HCL. The use of TrisTricine gel loading buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-Tricine-SDS gel running buffer. Tricine Sample Buffer does not contain reducing agent. For reducing conditions, add Mercaptoethanol or DL-DTT