Complete Supplement Mixture w/o URA, w/ 20 mg/l ADE

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SKU:
G113
Complete supplement for amino acids to prepare appropiate media for selection of URA3 auxotrophic strains and transformants


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Intended Use

Complete Supplement Mixture w/o URA, w/ 20mg/l ADE is used as a dropout supplement for all strains of Saccharomyces cerevisiae.

Composition

Ingredients Milligrams/Litre
Adenine 20.00
L-Arginine HCl 50.00
L-Aspartic acid 80.00
L-Histidine HCl 20.00
L-Isoleucine 50.00
L-Leucine 100.00
L-Lysine HCl 50.00
L-Methionine 20.00
L-Phenylalanine 50.00
L-Threonine 100.00
L-Tryptophan 50.00
L-Tyrosine 50.00
L-Valine 140.00

Formula adjusted, standardized to suit performance parameters

Directions

Suspend 780 mg in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.

Principle and Interpretation

Complete Supplement Mixture w/o URA, w/ 20mg/l ADE is used as a dropout supplement for all strains of Saccharomyces cerevisiae.

Quality Control

Appearance of Powder: Light yellow coloured, homogeneous, free flowing powder.

Colour and Clarity: Light yellow coloured, clear solution without any precipitate.

Cultural Response: Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.

Organisms (ATCC) Growth
Saccharomyces cerevisiae good-luxuriant

Storage and Shelf-life

Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.

More Information
Product Name Complete Supplement Mixture w/o URA, w/ 20 mg/l ADE
SKU G113
Customized Product Available No
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