Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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NZY (Harvard) Growth Medium
As low as
$148.50
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Availability: In stock
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SKU:
G028
Media for cultivation of recombinant Escherichia coli strains
Grams/Lit : 23.00 21.74 Lit/500G
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Escherichia coli#902790
NZY (Harvard) Growth Medium is used for cultivation of recombinant Escherichia coli strains.
Intended Use
NZY (Harvard) Growth Medium is used for cultivation of recombinant Escherichia coli strains.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Casein enzymic hydrolysate | 10.00 |
| Yeast extract | 5.00 |
| Sodium chloride | 8.00 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 23 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
NZY (Harvard) Growth Medium is used for cultivation of recombinant Escherichia coli strains. This medium was developed by Blattner and colleagues as a rich medium for the propagation of bacteriophages which infects its cell host, E. coli for replication (1). Cells grow very fast in this medium as this medium provides all the amino acids, vitamins and other metabolites required for cell growth (2). Casein enzymic hydrolysate provides nitrogen, amino acids, and carbon sources for the cells. Yeast extract functions as the source of vitamins and trace elements. Sodium chloride provides sodium ions for transport and osmotic balance (3). NZY (Harvard) Growth Medium allows the cells to grow more rapidly as they do have to synthesize nucleotide precursors and other factors required for growth.
Quality Control
Appearance of Powder : Cream to yellow coloured, homogeneous, free flowing powder.
Colour and Clarity
Light yellow coloured, clear solution without any precipitate.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 23724 | 50 - 100 | good-luxuriant |
| Escherichia coli ATCC 25922 | 50 - 100 | good-luxuriant |
| Escherichia coli MTCC 1652 | 50 - 100 | good-luxuriant |
Storage and Shelf-life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | NZY (Harvard) Growth Medium |
|---|---|
| SKU | G028 |
| Customized Product Available | No |
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