Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Modified Scholten's Broth
SKU:
M2165I
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for detection and enumeration of bacteriophages from water samples. The composition and performance criteria are as per specifications laid down in ISO 10705-2:2000.
g/L : 29.03 17.22 Lit/500G
Intended Use
Recommended for detection and enumeration of bacteriophages from water samples. The composition and performance criteria are as per specifications laid down in ISO 10705-2:2000.
Composition
| As per ISO 10705-2:2000 | Modified Scholten's Broth M2165I | ||
|---|---|---|---|
| Ingredients | g / L | Ingredients | g / L |
| Peptone | 10.000 | Peptone | 10.000 |
| Yeast extract | 3.000 | Yeast extract | 3.000 |
| Meat Extract | 12.000 | HM Extract# | 12.000 |
| Sodium chloride | 3.000 | Sodium chloride | 3.000 |
| Sodium carbonate (15%) | 5.00 ml | Sodium carbonate | 0.750 |
| Magnesium chloride (100 g in 50 ml water) | 0.30 ml | Magnesium chloride, hexahydrate | 0.600 |
| pH after sterilization | 7.2±0.5 | pH after sterilization | 7.2±0.5 |
**Formula adjusted, standardized to suit performance parameters.
# Equivalent to Meat extract.
Directions
Suspend 29.03 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml of purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes or flasks or as desired. Sterilize by autoclaving at 121±3°C for 15 minutes.
Principle and Interpretation
The formulation of Modified Scholten's Broth is as described in ISO 10705-2 (1). Bacteriophages, also known as phages, are viruses that infect and replicate only in bacterial cells. Like all viruses, bacteriophages are very species-specific with regard to their hosts and usually only infect a single bacterial species or even specific strains within a species. Phages are clinically significant for several reasons.
Peptone, HM Extract and yeast extract in the medium provides the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains the osmotic equilibrium of the medium. Magnesium chloride present in the medium raises the osmotic pressure. Sodium carbonate and magnesium chloride help to maintain the pH of the medium.
Type of Specimen
Water samples.
Specimen Collection and Handling
ISO 10705-2:2000 (1)
Rehydrate the content of a lyophilized ampoule of the reference culture of the host strain in 3 ml of Modified Scholten's Broth (M2165I). Transfer the suspension to 50 ml of Modified Scholten's Broth (M2165I) and incubate at 36±2°C for (20±4) h.
Add 1 ml of inoculum culture to each culture tube containing the aliquots of sample and Scholten's Agar (M2134I). Incubate the plates at 36±2°C for (18±2) h. Count the number of plaques on each plate within 4 h after finishing incubation, using indirect oblique light.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture.
Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder.
Colour and Clarity of prepared medium: Light amber colored clear to slightly opalescent solution.
Reaction: Reaction of 2.90% w/v aqueous solution at 25°C. pH after sterilization: 7.2±0.5.
pH after sterilization: 6.70-7.70.
Cultural Response: Cultural characteristics observed after an incubation at 36±2°C for (20±4) hours.
Technical Specifications
| Inoculum | Escherichia coli ATCC 25922 (00013*) |
| Inoculum (CFU) | 50-100 |
| Growth | good-Luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques
| Product Name | Modified Scholten's Broth |
|---|---|
| SKU | M2165I |
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